Team:KAIST Korea/Project Background

From 2012.igem.org

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<img id="starter-grad" style="height:60px" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
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<img src="https://static.igem.org/mediawiki/2012/9/9f/KAIST_Proj_Overview.png"/></br></br>
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        <h1>Overview</h1>
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Our new biobrick part <span style="color:#aa0000;"><b>FlipFlop(BBa_K907002 and BBa_K907003)</b> </span> is a noble device which can generate binary signal as its name tells us.</br></br>
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<span id="little">This part is composed of three sub-parts.</br></br></span>
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<li><b>Promoter</b> : <a href="링크걸어요">BBa_J23119</a></li>
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<li><b>RBS</b> : <a href="링크걸어요">BBa_B0034</a></li>
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<li><b>att sites</b> : Recognition site for <a href="링크걸어요">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase).</li>
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<div style="color:#0000aa;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 0</span></div>
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The FlipFlop, initially, promotes the transcription and translation of down-stream gene, Protein A in the Figure 2, due to its promoter orientation.
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When <a href="링크 걸어요">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.
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<div style="color:#CC0000;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 1</span></div>
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Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step), or Protein B(Signal 1) in Figure 4.</br></br>
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Also, the signal can be restored to its initial state when BBa_K907000/BBa_K907001 (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.</br>
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<div style="color:#0000aa;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 0</span></div>
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<img src="https://static.igem.org/mediawiki/2012/3/32/KAIST_Proj_Overview6.png"/>
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Revision as of 22:01, 26 September 2012

KAIST Korea 2012 iGEM

Project : Overview


Our new biobrick part FlipFlop(BBa_K907002 and BBa_K907003) is a noble device which can generate binary signal as its name tells us.

This part is composed of three sub-parts.
  1. Promoter : BBa_J23119
  2. RBS : BBa_B0034
  3. att sites : Recognition site for BBa_K907000(Mycobacteriophage Bxb1 integrase).


Signal 0

The FlipFlop, initially, promotes the transcription and translation of down-stream gene, Protein A in the Figure 2, due to its promoter orientation.

When BBa_K907000(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.


Signal 1

Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step), or Protein B(Signal 1) in Figure 4.


Also, the signal can be restored to its initial state when BBa_K907000/BBa_K907001 (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.


Signal 0
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