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Team:TU Darmstadt/Materials/PBS
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Latest revision as of 20:59, 26 September 2012
Contents |
PBS
About
PBS stands for phosphate buffered saline and is a isotonic buffer solution.
Procedure
For 1x PBS
- Combine:
- 8.18 g NaCl (140 mM)
- 0.2 g KCl (2.7 mM)
- 1.77 g Na2HPO4 (10 mM)
- 0.24 g KH2PO4 (1.8 mM)
- Add. 1L dH2O
- Adjust to pH 7.4
For 10x PBS
- Combine the following:
- 80 g NaCl
- 2 g KCl
- 14.4 g Na2HPO4 (dibasic anhydrous) OR 18.1 g Na2HPO4 · 2H2O (dibasic dihydrate) OR 27.2 g Na2HPO4 · 7H2O (dibasic heptahydrate)
- 2.4 g KH2PO4 (monobasic anhydrous)
- 800 mL distilled H2O
- Adjust pH to 7.4 with HCl
- Add H2O to 1L
- Autoclave for 20 minutes on liquid cycle. Store at RT.[1]
References
[1] http://openwetware.org/wiki/PBS
