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Team:TU Darmstadt/Materials/Kanamycin
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
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=== About === | === About === | ||
Kanamycin is an aminoglycoside [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that interacts with the 30S subunit of prokayotic ribosomes inducing mistranslations thus inhibiting translocation during protein synthesis.<sup>[1]</sup> | Kanamycin is an aminoglycoside [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that interacts with the 30S subunit of prokayotic ribosomes inducing mistranslations thus inhibiting translocation during protein synthesis.<sup>[1]</sup> | ||
| + | === Stock === | ||
| + | # Mix: | ||
| + | #* 3 g kanamycin (75 mg/mL) | ||
| + | #* ad. 40 mL dist. water | ||
| + | # sterile filtration | ||
| + | # aliquot in 1 mL stocks and store at -20°C | ||
=== Stability === | === Stability === | ||
Latest revision as of 20:42, 26 September 2012
Contents |
Kanamycin
About
Kanamycin is an aminoglycoside antibiotic that interacts with the 30S subunit of prokayotic ribosomes inducing mistranslations thus inhibiting translocation during protein synthesis.[1]
Stock
- Mix:
- 3 g kanamycin (75 mg/mL)
- ad. 40 mL dist. water
- sterile filtration
- aliquot in 1 mL stocks and store at -20°C
Stability
- Kanamycin is one of the few autoclavable antibiotics
- Culture plates can be stored for about 2-3 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])
References
[1] Pestka, S.: "The Use of Inhibitors in Studies on Protein Synthesis", Methods in Enzymology 30, pp.261-282, 1975
