Team:HKUST-Hong Kong/Project Abstraction

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         <div id="Navigation_top">
         <div id="Navigation_top">
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           <div id="iGEM_Logo" class="Upper_Logos"></div>
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           <a href="https://2012.igem.org/Main_Page"><img id="iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/3/32/Igem-logo.png"></a>
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           <div id="HKUST_Logo" class="Upper_Logos"></div>
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           <a href="http://www.ust.hk"><img id="HKUST_Logo" class="Upper_Logos" src=""></a>
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          <a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><img id="HKUST_iGEM_Logo" class="Upper_Logos" src="https://static.igem.org/mediawiki/2012/4/43/295197_446935765350523_472857901_n.jpg"></a>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Team" align="center"><h3><p>TEAM</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
           <div class="Navigation_Buttons" id="Project" align="center"><h3><p>PROJECT</p></h3></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></div>
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              <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
 
           </div>
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           <div class="Navigation_Content" id="Project_Content">
           <div class="Navigation_Content" id="Project_Content">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Project Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Background and<br> Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project">Project Description</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module">Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Chassis">Chassis</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Expectation">Expectation</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Potential_Application">Potential application</a></p></div>
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           </div>
           </div>
           <div class="Navigation_Content" id="Wet_lab_Content" align="center">
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Prospect">Prospect</a></p></div>
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               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></div>
           </div>
           </div>
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
           <div class="Navigation_Content" id="Human_practice_Content" align="center">
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           </div>
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           <div class="Navigation_Content" id="Extras_Content" align="center">
           <div class="Navigation_Content" id="Extras_Content" align="center">
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></div>
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                <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></div>
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                <div class="Content_Buttons""><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
               <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></div>
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Site_Map">Site Map</a></p></div>
 
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              <div class="Content_Buttons"><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Photo_Gallery">Photo Gallery</a></p></div>
 
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<div><p align="center"><font size="20">PROJECT ABSTRACT</font></p></div>
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<div><p align="center"><font size="20">Project Abstract</font></p></div>
           <div id="paragraph1" class="bodyParagraphs">
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               <h1><p align=center>Project name: B. hercules <br>---<i style="size:15px">The Terminator of Colon Cancer</i></p></h1>
               <h1><p align=center>Project name: B. hercules <br>---<i style="size:15px">The Terminator of Colon Cancer</i></p></h1>
           </div>
           </div>
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           <p>The possible adverse effects of dispersing toxic anti-tumor agents in the circulatory system during conventional cancer treatment prompt us to consider the need of an alternative cancer therapeutic method. In an effort to combat colorectal carcinoma, we aim to use genetically modified <i>Bacillus subtilis</i> to execute targeted drug delivery to cancer cells in the lower digestive tract, offering the advantage of generating minimal negative effects on normal colon epithelial cells. Targeting is achieved by expressing RPMrel, a colon tumor specific binding peptide, on the cell wall of <i>Bacillus subtilis</i> using a LytC cell wall binding system. Upon successful binding, the anti-tumor cytokine bone morphogenetic protein 2(BMP-2), can be synthesized and secreted from the bacterial cells with the help of a signalling peptide fused to the protein. Minimization of the harmful effect of BMP2 overdose is made possible by introducing two regulatory systems: xylose-inducible system and <i>ydcE/ydcD</i> toxin-antitoxin system, which serve to control the timing and amount of BMP2 release. </p>
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           <p>The possible adverse effects of dispersing toxic anti-tumor agents in the circulatory system during conventional cancer treatment prompt us to consider the need of an alternative cancer therapeutic method. In an effort to combat colorectal carcinoma, we aim to use genetically modified <i>Bacillus subtilis</i> to execute targeted drug delivery to cancer cells in the lower digestive tract, offering the advantage of generating minimal negative effects on normal colon epithelial cells. Targeting is achieved by anchoring a colon tumor-specific binding peptide, RPMrel, onto the cell wall of <i>Bacillus subtilis</i> using a LytC cell wall binding system. Upon successful binding, the anti-tumor cytokine bone morphogenetic protein 2 (BMP2), can be synthesized and secreted from the bacterial cells with the help of a signalling peptide fused to the protein. Minimization of the harmful effect of BMP2 overdose is made possible by introducing two regulatory systems: xylose-inducible system and <i>ydcE/ydcD</i> toxin-antitoxin system, which serve to control the timing and amount of BMP2 release. </p>
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<p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong"><b>Home</b></a></p>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Team</b><p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Introduction">Introduction</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Supervisor">Supervisor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Instructor">Instructor</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Members">Members</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Advisors">Advisors</a></p></li>
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</ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Project</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction">Abstract</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Background_and_Motive">Motive</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Overview">Design - Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Module">Design - Module</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Target_binding">Target Binding Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Anti_tumor">Anti-tumor Molecule Secretion Module</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Module/Regulation_and_control">Regulation and Control Module</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Design_Chassis">Design - Chassis</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Wet Lab</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Overview</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Construction">Construction</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Assembly">Assembly</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Logbook">Logbook</a></p>
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<p>-- <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook/Protocol">Protocol</a></p>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Achievement">Achievement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Future_Work">Future Work</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Human Practice</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Overview">Overview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Interview">Interview</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Presentation">Presentation</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Calendar">Calendar</a></p></li></ol>
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</div>
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<div class="Sitemap_Content">
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<li><p><b>Extras</b></p><ol>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Medal_Requirements">Medal Requirements</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety">Safety</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Attribution">Attribution</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Acknowledgement">Acknowledgement</a></p></li>
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<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Glossary">Glossary</a></p></li></ol>
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</div>
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Latest revision as of 19:02, 26 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Project Abstract

Project name: B. hercules
---The Terminator of Colon Cancer

The possible adverse effects of dispersing toxic anti-tumor agents in the circulatory system during conventional cancer treatment prompt us to consider the need of an alternative cancer therapeutic method. In an effort to combat colorectal carcinoma, we aim to use genetically modified Bacillus subtilis to execute targeted drug delivery to cancer cells in the lower digestive tract, offering the advantage of generating minimal negative effects on normal colon epithelial cells. Targeting is achieved by anchoring a colon tumor-specific binding peptide, RPMrel, onto the cell wall of Bacillus subtilis using a LytC cell wall binding system. Upon successful binding, the anti-tumor cytokine bone morphogenetic protein 2 (BMP2), can be synthesized and secreted from the bacterial cells with the help of a signalling peptide fused to the protein. Minimization of the harmful effect of BMP2 overdose is made possible by introducing two regulatory systems: xylose-inducible system and ydcE/ydcD toxin-antitoxin system, which serve to control the timing and amount of BMP2 release.

Retrieved from "http://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction"