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Team:TU Darmstadt/Protocols/Bacterial transformation
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There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are: | There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are: | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | ||
| - | and | + | and |
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | ||
The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells. | The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells. | ||
| - | === Application == | + | === Application === |
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | ||
| - | == References == | + | === References === |
* Praktikumsskript Biotechnologie 2012 TU Darmstadt | * Praktikumsskript Biotechnologie 2012 TU Darmstadt | ||
Latest revision as of 18:41, 26 September 2012
Bacterial transformation
There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are:
and
The transformation of E. coli with plasmid DNA according to those methods do require chemically/electro-competent cells.
Application
References
- Praktikumsskript Biotechnologie 2012 TU Darmstadt
