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Team:UT-Tokyo/LabWork/AssayMethods
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Gas Chromatography is a method used for separating and analyzing compounds by injecting ａ sample mixture into a column prepared to be in a suitable stationary phase and passing a gas （carrier gas） in the mobile phase through the column, in order to separate the mixture into its components by making use of the difference in retention capacity against the stationary phase. This method can be applied to a gaseous or vaporizable sample, and is used for identification, purity testing, and quantitative determination. | Gas Chromatography is a method used for separating and analyzing compounds by injecting ａ sample mixture into a column prepared to be in a suitable stationary phase and passing a gas （carrier gas） in the mobile phase through the column, in order to separate the mixture into its components by making use of the difference in retention capacity against the stationary phase. This method can be applied to a gaseous or vaporizable sample, and is used for identification, purity testing, and quantitative determination. | ||
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| + | ===Materials=== | ||
| + | |||
| + | *Gas Chromatograph ; SHIMADZU GC-8A with a thermal conductivity detector | ||
| + | |||
| + | column ; molecular sieve 13X 60/80 | ||
| + | |||
| + | carrer gas ; nitrogen at 30 mL/min | ||
| + | |||
| + | injection temperature ; 50℃ | ||
| + | |||
| + | column temperature ; 42℃ | ||
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| + | current ； 90 mA | ||
| + | |||
| + | *0.5 mL micro-syringe | ||
| + | *2 mL vial | ||
| + | *single colony of bacteria containing our construct that raises hydrogen production; | ||
| + | FhlA ; Plac－RBS－FhlA-d.term | ||
| + | |||
| + | m-FhlA ; Plac－RBS－(m-FhlA)－d.term | ||
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Revision as of 16:53, 26 September 2012
Assay Methods
Methods for Assay
H2 detection and assay
Gas Chromatography
Gas Chromatography is a method used for separating and analyzing compounds by injecting ａ sample mixture into a column prepared to be in a suitable stationary phase and passing a gas （carrier gas） in the mobile phase through the column, in order to separate the mixture into its components by making use of the difference in retention capacity against the stationary phase. This method can be applied to a gaseous or vaporizable sample, and is used for identification, purity testing, and quantitative determination.
Materials
- Gas Chromatograph ; SHIMADZU GC-8A with a thermal conductivity detector
column ; molecular sieve 13X 60/80
carrer gas ; nitrogen at 30 mL/min
injection temperature ; 50℃
column temperature ; 42℃
current ； 90 mA
- 0.5 mL micro-syringe
- 2 mL vial
- single colony of bacteria containing our construct that raises hydrogen production;
FhlA ; Plac－RBS－FhlA-d.term
m-FhlA ; Plac－RBS－(m-FhlA)－d.term
