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Team:Freiburg/Project
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<p><div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> | <p><div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> | ||
You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">overview of this exciting new field of research.</div> | You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">overview of this exciting new field of research.</div> | ||
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== [[Team:Freiburg/Project/Overview|1. Golden Gate Standard]]== | == [[Team:Freiburg/Project/Overview|1. Golden Gate Standard]]== | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">therefore introduce the new Golden Gate <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Standard that is fully compatible with RFC 10.</div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">therefore introduce the new Golden Gate <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Standard that is fully compatible with RFC 10.</div> | ||
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==[[Team:Freiburg/Project/Vektor|2. The TAL Vector]]== | ==[[Team:Freiburg/Project/Vektor|2. The TAL Vector]]== | ||
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<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">need to actually work with DNA.To make it even more easy these tools are deliverd already inside the </div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">need to actually work with DNA.To make it even more easy these tools are deliverd already inside the </div> | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">final TAL backbone, just add the sequence and you're ready.</div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">final TAL backbone, just add the sequence and you're ready.</div> | ||
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== [[Team:Freiburg/Project/Overview|3. GATE Assembly Kit]]== | == [[Team:Freiburg/Project/Overview|3. GATE Assembly Kit]]== | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">the theory behind the TAL effector toolkit, how we created it and why we choose this design.</div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">the theory behind the TAL effector toolkit, how we created it and why we choose this design.</div> | ||
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==[[Team:Freiburg/Project/Tal|4. Using the Toolkit]]== | ==[[Team:Freiburg/Project/Tal|4. Using the Toolkit]]== | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Our overall goal is to empower future iGEM teams to use the most exciting new technology synthetic biology has to offer. We therefore not only invented the GATE assembly platform but wrote a step by step manual for super-easy custom TALE construction | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Our overall goal is to empower future iGEM teams to use the most exciting new technology synthetic biology has to offer. We therefore not only invented the GATE assembly platform but wrote a step by step manual for super-easy custom TALE construction | ||
</div> | </div> | ||
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==[[Team:Freiburg/Project/Robot|4. The future TAL projects]]== | ==[[Team:Freiburg/Project/Robot|4. The future TAL projects]]== | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Until now, almost three years after deciphering the TALE code, only two types of TAL Effectors have been developed: TALENs and TAL-TFs. We herein propose additional classes of TAL effectors. | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Until now, almost three years after deciphering the TALE code, only two types of TAL Effectors have been developed: TALENs and TAL-TFs. We herein propose additional classes of TAL effectors. | ||
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==[[Team:Freiburg/Project/Experiments|5. Experiments and Results]]== | ==[[Team:Freiburg/Project/Experiments|5. Experiments and Results]]== | ||
We not only rigorously tested if our in vitro TALE gene assembly method works but also if our TALE constructs actually work in a human cell line. Check out test design and results. | We not only rigorously tested if our in vitro TALE gene assembly method works but also if our TALE constructs actually work in a human cell line. Check out test design and results. | ||
Revision as of 07:32, 26 September 2012
Project
Overall project
Since June 2012, we are working in the field of so called TAL effectors in our lab. TAL effectors are molecular components that are fused to DNA binding proteins in order to restrict their acticvity to specific loci in a genome. In particular, we develop a strategy to cheaply create novel TAL effectors in an extremely short period of time. We believe that we have laid a solid foundation for super-easy site specific genome modifications by future iGEM teams.
We hope you will learn something about our project and the power of TAL effectors.
0. Introduction
You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick
overview of this exciting new field of research.
1. Golden Gate Standard
Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We
therefore introduce the new Golden Gate
Standard that is fully compatible with RFC 10.
2. The TAL Vector
Targeting a sequence and not doing something to it, is like throwing mechanics at your car. Your car will
not get any better only the mechanics will get mad. Because we know this, we bring the tools you
need to actually work with DNA.To make it even more easy these tools are deliverd already inside the
final TAL backbone, just add the sequence and you're ready.
3. GATE Assembly Kit
We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about
the theory behind the TAL effector toolkit, how we created it and why we choose this design.
4. Using the Toolkit
Our overall goal is to empower future iGEM teams to use the most exciting new technology synthetic biology has to offer. We therefore not only invented the GATE assembly platform but wrote a step by step manual for super-easy custom TALE construction
4. The future TAL projects
Until now, almost three years after deciphering the TALE code, only two types of TAL Effectors have been developed: TALENs and TAL-TFs. We herein propose additional classes of TAL effectors.
5. Experiments and Results
We not only rigorously tested if our in vitro TALE gene assembly method works but also if our TALE constructs actually work in a human cell line. Check out test design and results.
