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Team:Slovenia/ModelingMutualRepressorSwitchStochastic
From 2012.igem.org
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<h2><a name="model">The model</a></h2> | <h2><a name="model">The model</a></h2> | ||
| + | <p>The basis for the stochastic simulation of the mutual repressor switch was the following set of reactions that describe the dynamics of the switch:</p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/2/22/Svn12_mrs_stoh1.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/b/b6/Svn12_mrs_stoh2.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/d/d0/Svn12_mrs_stoh3.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/d/da/Svn12_mrs_stoh4.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/1/19/Svn12_mrs_stoh5.PNG" /></p> | ||
| + | <p><img src=https://static.igem.org/mediawiki/2012/7/73/Svn12_mrs_stoh6.PNG"" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/1/10/Svn12_mrs_stoh7.PNG" /></p> | ||
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Revision as of 22:33, 25 September 2012
Modeling - mutual repressor switch
- Deterministic model
- Stochastic model
- C#Sim model
Stochastic model of the mutual repressor switch
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Stochastic simulation revealed that the mutual repressor switch exhibited bistability only as long as there was no leaky expression present. Otherwise, cooperativity higher than 1 was required. Higher cooperativity improved leaky expression tolerance, but only to a certain threshold. Higher leaky expression resulted in lower stable-state levels. Bistable behavior of the switch became inconsistent when a delay between transcription and translation was taken into account. |
The model
The basis for the stochastic simulation of the mutual repressor switch was the following set of reactions that describe the dynamics of the switch:
![](https://static.igem.org/mediawiki/2012/7/73/Svn12_mrs_stoh6.PNG"")
Simulation results
Next: C#Sim model of the mutual repressor switch >>
