As part of our <i>Synechocystis</i> transformation strategies we devised using psb1C3_IntC plasmid developed by 2010 iGEM Utah team. We sent them an e-mail asking them for DNA which they sent to us.
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<h1>Peter Lindblad - Uppsala University</h1>
<h1>Peter Lindblad - Uppsala University</h1>
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At the end of March we sent an e-mail to professor Voigt asking him for spider siLK monomers ADF-1,2,3 that his team used in the paper: "Engineering the <i>Salmonella</i> type III secretion system to export spider silk monomers". We planned to use those sequences to build spider silk biobricks and to engineer <i>E. coli</i> to secrete these monomers.
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At the end of March we sent an e-mail to professor Voigt asking him for spider silk monomers ADF-1,2,3 that his team used in the paper: "Engineering the <i>Salmonella</i> type III secretion system to export spider silk monomers". We planned to use those sequences to build spider silk biobricks and to engineer <i>E. coli</i> to secrete these monomers.
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<h1>TU_Munich 2012 iGEM team</h1>
<h1>TU_Munich 2012 iGEM team</h1>
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Revision as of 00:51, 25 September 2012
Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012
As part of our Synechocystis transformation strategies we devised using psb1C3_IntC plasmid developed by 2010 iGEM Utah team. We sent them an e-mail asking them for DNA which they sent to us.
Peter Lindblad - Uppsala University
Dr. Lindblad is the head of a research group that has published about molecular tools for synthetic biology in cyanobacteria.
When we were planning transformation strategies for Synechocystis we found literature about pPMQAK1 plasmid developed by his team.
By February we contacted Dr. Lindblad and asked him for pPMQAK1 DNA which he kindly sent to us.
Chris Voigt - MIT
At the end of March we sent an e-mail to professor Voigt asking him for spider silk monomers ADF-1,2,3 that his team used in the paper: "Engineering the Salmonella type III secretion system to export spider silk monomers". We planned to use those sequences to build spider silk biobricks and to engineer E. coli to secrete these monomers.