Team:SDU-Denmark/labwork/Protocols/Checkdigest

From 2012.igem.org

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<b>Digest</b></br></br>
<b>Digest</b></br></br>
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1) Cast an agarose gel for purification of the cut product</br></br>
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1)Cast an agarose gel for purification of the cut product</br></br>
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2) Mix the digestion mixture in an eppendorf tube</br></br>
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2)Mix the digestion mixture in an eppendorf tube</br></br>
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3) Leave for 5 min. at 37°C (no shaking!)</br></br>
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3)Leave for 5 min. at 37°C (no shaking!)</br></br>
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4) Immidiately load the restriction mixture in the gel</br></br>
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4)Immidiately load the restriction mixture in the gel</br></br>
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5) Run the gel, cut out and purify bands of correct size.</br></br>
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5)Run the gel, cut out and purify bands of correct size.</br></br>
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</br>
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Revision as of 00:06, 25 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID





mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenisis PCR-,gel clean-up Content

Check Digest

Digest mix, using Fastdigest enzymes


Digest

1) Cast an agarose gel for purification of the cut product

2) Mix the digestion mixture in an eppendorf tube

3) Leave for 5 min. at 37°C (no shaking!)

4) Immidiately load the restriction mixture in the gel

5) Run the gel, cut out and purify bands of correct size.


Digestion Mix

4 µL H2O 
0,5 µL enzyme A
0,5 µL enzyme B
2 µL Fast Digest green buffer
3 µL dna product


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