Team:SDU-Denmark/labwork/Protocols/Checkdigest

From 2012.igem.org

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<h2>Digest mix, using Fastdigest enzymes</h2>
<h2>Digest mix, using Fastdigest enzymes</h2>
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Digestion Mix
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<b>Digest</b></br></br>
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1)Cast an agarose gel for purification of the cut product</br></br>
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2)Mix the digestion mixture in an eppendorf tube</br></br>
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3)Leave for 5 min. at 37°C (no shaking!)</br></br>
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4)Immidiately load the restriction mixture in the gel</br></br>
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5)Run the gel, cut out and purify bands of correct size.</br></br>
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</br>
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<b>Digestion Mix</b></br>
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</br>
</br>
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Digest</br>
 
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1)Cast an agarose gel for purification of the cut product</br></br>
 
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2)Mix the restriction mixture in an eppendorf tube</br></br>
 
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3)Leave for 5 min. at 37°C (no shaking!)</br></br>
 
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4)Immidiately load the restriction mixture in the gel</br></br>
 
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5)Run the gel, cut out and purify bands of correct size.</br></br>
 
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Revision as of 00:04, 25 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID





mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenisis PCR-,gel clean-up Content

Check Digest

Digest mix, using Fastdigest enzymes


Digest

1)Cast an agarose gel for purification of the cut product

2)Mix the digestion mixture in an eppendorf tube

3)Leave for 5 min. at 37°C (no shaking!)

4)Immidiately load the restriction mixture in the gel

5)Run the gel, cut out and purify bands of correct size.


Digestion Mix
4 µL H2O 
0,5 µL enzyme A
0,5 µL enzyme B
2 µL Fast Digest green buffer
3 µL dna product


Retrieved from "http://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/Checkdigest"