Team:UC Chile2/Cyanolux Protocols

From 2012.igem.org

(Difference between revisions)
(→Methods for characterization of LuxBrick (K325909))
 
(2 intermediate revisions not shown)
Line 1: Line 1:
{{UC_Chile4}}
{{UC_Chile4}}
-
<div ="primarycontent">
+
{{UC_Chilefooter}}
-
 
+
-
== Methods for characterization of LuxBrick (K325909) ==
+
-
<font face = "Calibri">
+
-
 
+
-
* Pick a single colony from a transformed plate of K325909. Grow in LB media with chloramphenicol 30ug/mL O/N at 30°C.
+
-
 
+
-
* Measure OD at 600nm, then dilute to OD600=0.6 following direct C*V ratio to obtain bacterial stock for experiments.
+
-
 
+
-
* Induce with 3mM (L)-Arabinose (final concentration).
+
-
 
+
-
* Alicuot 600uL of bacteria and add LB media with chloramphenicol 30ug/mL + additives and inducers up to 1.5mL. This will yield a 0.24 OD600nm.
+
-
 
+
-
* Grow at 30°C for 1.5 hours (or at chosen T° condition for characterization).
+
-
 
+
-
* Alicuot 100uL into 4 wells (at least) of a 96 well falcon plate (plaque?).
+
-
 
+
-
* Measure luminescence in a ThermoScientific Luminoskan Ascent Microplate Luminometer.
+
-
</font>
+
-
</div>
+

Latest revision as of 05:45, 21 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012