Team:Goettingen/week21-2

From 2012.igem.org

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<b> V09_18_1 Motility Assays</i></i></b><br>
<b> V09_18_1 Motility Assays</i></i></b><br>
<ul>
<ul>
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<li>Experiment: <br>  
+
<li>Experiment: For the motility assay the over night cultures, with different constructs, were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">HERE</a> <br>
 +
<li> Observation and Results:<br>
Text<br>
Text<br>
</ul>
</ul>
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</ul>
</ul>
<br>
<br>
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<b>V09_18_3 Chemical transformation of</b><br>
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<b>V09_18_3 Chemical transformation of different plasmid-gene constructs into <i>E. coli</i> (DH10B) or (MG1655)</b><br>
 +
<ul>
 +
<li>Experiment:<br> Chemical transformation was done according to the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The following constructs were used:<br>
 +
- pSB1C3-<i>FliC</i> into <i>E. coli </i>(DH10B)<br>
 +
pSB1C3-<i>flhDC</i> into <i>E. coli </i>(DH10B)<br>
 +
PSB1C3-<i>RFP</i> into <i>E. coli </i> (MG1655)<br>
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<br>
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<br></td></tr>
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</table>
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<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
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<tr bordercolor="black" valign="top">
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<td width="900" bordercolor="black" valign="top">
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<h2><b>V09_18 </b></h2><br>
 +
<b> V09_18_1 Motility Assays</i></i></b><br>
 +
<ul>
 +
<li>Experiment: For the motility assay the over night cultures, with different constructs, were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">HERE</a> <br>
 +
<li> Observation and Results:<br>
 +
Text<br>
 +
</ul>
 +
<br>
 +
<b>V09_18_2 Preparative double digestion of <i>MotA</i>, <i>MotB</i>, <i>yhjH</i>, 18C-<i>RFP</i>, 20E-<i>RFP</i> and 20I-<i>RFP</i> followed by ligation</i></b><br>
<ul>
<ul>
<li>Experiment: <br>
<li>Experiment: <br>
-
TEXT<br>
+
In order to test the influence of the different genes, we cloned the genes behind constitutive promoters with various promoter strength. <i> MotA, MotB</i> and <i>yhjH</i> were digested with XbaI and PstI. The psB1C3-Promoter backbones were digested with SpeI and PstI. Afterwards, the fragments of the desired size were cut out and purified via gel-extraction kit. Finally, over night ligation was started.<br>
-
 
+
</ul>
 +
<br>
 +
<b>V09_18_3 Chemical transformation of different plasmid-gene constructs into <i>E. coli</i> (DH10B) or (MG1655)</b><br>
 +
<ul>
 +
<li>Experiment:<br> Chemical transformation was done according to the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The following constructs were used:<br>
 +
- pSB1C3-<i>FliC</i> into <i>E. coli </i>(DH10B)<br>
 +
pSB1C3-<i>flhDC</i> into <i>E. coli </i>(DH10B)<br>
 +
PSB1C3-<i>RFP</i> into <i>E. coli </i> (MG1655)<br>
 +
<br>
<br></td></tr>
<br></td></tr>
</table>
</table>

Revision as of 09:50, 19 September 2012

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