Team:Goettingen/week18-2
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<h2><b>V08_27 </b></h2><br> | <h2><b>V08_27 </b></h2><br> | ||
- | <b> | + | <b>V08_27_1 Miniprep of the <i>tar</i>-promoter constructs</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
- | + | The Minipreps were conducted using the PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br></li> | |
- | </ul> | + | <br> |
- | <br></td></tr> | + | <b>V08_27_2 Chemical retransformation of the <i>tar</i>-promoter constructs into <i>E. coli</i> BL21 </b><br> |
+ | <ul> | ||
+ | <li>Experiment: <br> | ||
+ | The retransformation was performed as described in the standard protocol.<br> | ||
+ | <li>Observations & Results: <br> | ||
+ | </li> | ||
+ | </ul><br> | ||
+ | <b>V08_27_3 Chemical transformation of different motility increasing constructs into <i>E. coli</i> MG1655</b><br> | ||
+ | <ul> | ||
+ | <li>Experiment: <br> | ||
+ | For we aimed to investigate the effect of our constructs on cells that are used to swim competent MG1655 cells were transformed with the following constructs: <br> | ||
+ | <i>fliC</i> (DH10B) <br> | ||
+ | <i>fliC</i> (<i>Salmonella</i>) <br> | ||
+ | <i>motA</i> <br> | ||
+ | <i>motB</i> <br> | ||
+ | <i>yhjH</i> <br> | ||
+ | <br> | ||
+ | 18M-<i>flhDC</i> <br> | ||
+ | 18O-<i>flhDC</i> <br> | ||
+ | 18C-<i>flhDC</i></li> | ||
+ | <li>Observations & Results: <br> | ||
+ | </li> | ||
+ | |||
+ | </ul><br> | ||
+ | </td></tr> | ||
</table> | </table> | ||
<br> | <br> | ||
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Revision as of 10:44, 18 September 2012
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