Team:Goettingen/week21-2

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Revision as of 07:42, 18 September 2012

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V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and pSB1C3 followed by ligation

Experiment:
In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .

V09_17_2 Preparation of over night cultures

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 <b>V09_17_2 Preparation of over night cultures</b><br>
           <ul>
-<li>Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used: <br>
+<li>Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (MG1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used: <br>
 <br>
 - 20E_<i>flhDC</i> <br>