Team:Goettingen/week21-2

From 2012.igem.org

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<h2><b>V09_17 </b></h2><br>
<h2><b>V09_17 </b></h2><br>
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<b>Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation</i></b><br>
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<b>V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation</i></b><br>
<ul>
<ul>
<li>Experiment: <br>  
<li>Experiment: <br>  
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In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
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In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a> href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
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</li>
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<b>V09_17_2 Preparation of over night cultures</b><br>
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        <ul>
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<li>Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used: <br>
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- 20E_flhDC <br>
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- 18C_flhDC <br>
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- 20I_flhdc <br>
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- 20E_motA <br>
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- 18C_motA <br>
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- 20E motB <br>
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<h2><b>Versuchsnummer </b></h2><br>
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- 18C_motB <br>
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<b>TITEL</b><br>
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<ul>
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<li>Experiment: <br>
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Text</li>
Text</li>
</li></ul>
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Revision as of 07:35, 18 September 2012

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