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Team:Goettingen/week8-3
From 2012.igem.org
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<b>V06_21_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br> | <b>V06_21_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br> | ||
<ul> | <ul> | ||
| - | <li>Experiment: <br>All 8 promoter constructs + TAR_QC in | + | <li>Experiment: <br>All 8 promoter constructs + TAR_QC in J61002 and the BioBrick vector pSB1C3 were digested with <i>Xba</i>I and <i>Pst</i>I according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol. </a></li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
| - | <li>Observations and results: <br>The transformation of DH10B with pSB1C3 (see V06_18) worked well. | + | <li>Observations and results: <br>The transformation of DH10B with pSB1C3 (see V06_18) worked well. pSB1C3 contained RFP which is now cut out. |
</li> | </li> | ||
</ul> | </ul> | ||
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</ul> | </ul> | ||
<ul> | <ul> | ||
| + | <br></td></tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
| + | <tr bordercolor="black" valign="top"> | ||
| + | <td width="900" bordercolor="black" valign="top"> | ||
| + | <h2><b>V06_22 </b></h2><br> | ||
| + | <b>Isolation of vector backbone pSB1C3 from BioBrick distribution kit</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br>The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed <i>E. coli</i> DH10B with pSB1C3 according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> and streaked these out on Chloramphenicol (CM) plates.</li> | ||
| + | </ul> | ||
<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
Revision as of 11:49, 17 September 2012
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