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Team:Goettingen/week10-2
From 2012.igem.org
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| - | <h2><b><a name=" | + | <h2><b><a name="week10#2">#2 Speed Improvement - 10th week</a></b></h2> |
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | ||
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<table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
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| - | <h2><b> | + | |
| - | <b> | + | <h2><b>V06_11 </b></h2><br> |
| - | <ul> | + | <b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> |
| - | <li>Experiment: <br> | + | |
| - | + | <ul> | |
| + | <li>Experiment: <br> | ||
| + | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | ||
| + | |||
| + | </ul> | ||
<br> | <br> | ||
| - | </li> | + | |
| + | |||
| + | <b>V06_11_2 Chemical retransformartion of the new <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into <i>E. coli</i>:<br> | ||
| + | 20G - #1 <br> | ||
| + | 20G - #2 <br> | ||
| + | 2G - #1 <br> | ||
| + | 2G - #2 <br> | ||
| + | 2G - #3 <br> | ||
| + | 20I - #2 <br> | ||
| + | 20I - #3 <br> | ||
| + | 18O - #2 <br> | ||
| + | 18O - #3 <br> | ||
| + | 18K - #1 <br> | ||
| + | 18K - #2 <br> | ||
| + | 18K - #3 <br></li> | ||
| + | <li>Observations & Results: <br> | ||
| + | The retransformation was successful since all plates showed numeours colonoes except the negative control. | ||
| + | </ul> | ||
| + | |||
| + | |||
| + | <br></td></tr> | ||
</table> | </table> | ||
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<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
| + | <h2><b>V06_12 </b></h2><br> | ||
| + | <b>Preparation of over night cultures</i></b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br> | ||
| + | 20E - #2 <br> | ||
| + | 20G - #1 <br> | ||
| + | 2G - #1 <br> | ||
| + | 20I - #2 <br> | ||
| + | 18M - #2 <br> | ||
| + | 18O - #2 <br> | ||
| + | 18K - #1 <br> | ||
| + | 18C - #2 <br> | ||
| + | <br></td></tr> | ||
| + | </table> | ||
| - | <h2><b> | + | <br> |
| - | <b> | + | |
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
| + | <tr bordercolor="black" valign="top"> | ||
| + | <td width="900" bordercolor="black" valign="top"> | ||
| + | |||
| + | <h2><b>V06_13 </b></h2><br> | ||
| + | <b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> | ||
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | + | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li></ul> | |
| - | </li></ul> | + | |
<br> | <br> | ||
| + | <b>V06_13_2 Chemical transformartion of pBAD-<i>sfGFP</i> into <i>E. coli</i> (DH10B)</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | For the chemical transformation the standard protocol was followed. <br> | ||
| + | <li>Observations & Results: <br> | ||
| + | The transformation was successful since all plates showed numeours colonoes except the negative control.</li> | ||
| + | </ul><br> | ||
| + | |||
</td></tr> | </td></tr> | ||
</table> | </table> | ||
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<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
| - | <h2><b> | + | <h2><b>V06_14 </b></h2><br> |
| - | <b> | + | <b> Preparation of over night cultures</i></i></b><br> |
<ul> | <ul> | ||
| - | <li>Experiment: <br> | + | <li>Experiment: <br> |
| - | + | Over night cultures of the pBAD-<i>sfGFP</i> clones were prepared in order to isolate the plasmids. <br> | |
| + | <br></td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
| + | <tr bordercolor="black" valign="top"> | ||
| + | <td width="900" bordercolor="black" valign="top"> | ||
| + | <h2><b>V06_15 </b></h2><br> | ||
| + | <b> Miniprep of pBAD-<i>sfGFP</i></i></i></b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br> | ||
<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
Revision as of 14:19, 16 September 2012
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