Team:Goettingen/week19-2

V09_03

• Experiment:
  Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").
  
• Observations & Results:
  The QC PCR was not successful. We were not able to observe any PCR product.

V09_04

• Experiment:
  The test digestion of pSB1C3 including flHDC under the expression of 8 different promoters was performed with the restriction enzymes EcoRI and PstI according to the protocol. The product was subsequently analyzed on an 1% analytical agarose gel.
  
• Observations & Results:
  Since fragments of the expected size could be obtained for all clones the test digestion worked for all.

• Experiment:
  PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).

V09_05

• Experiment:
  motA, motB, yhjH and pSB1C3 were digested with EcoRI and PstI. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).

• Experiment:
  Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").
  
• Observations & Results:
  The QC PCR was not successful again. We were not able to observe any PCR product.

V09_06

• Experiment:
  After the ligation of motA, motB and yhjH into the plasmid pSB1C3, the ligation products were transformed into the E. coli strain DH10B.

V09_09

• Experiment:
  Over night cultures were prepared:
  pSB1C3-motA
  pSB1C3-motB