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Team:Goettingen/week20-2
From 2012.igem.org
(Difference between revisions)
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<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
<h2><b>V09_10 </b></h2><br> | <h2><b>V09_10 </b></h2><br> | ||
| - | <b>V09_10_1 Mini Prep of Psb1c3 (including motA | + | <b>V09_10_1 Mini Prep of Psb1c3 (including motA, MotB and yhjH)</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li> | <li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li> | ||
</ul> | </ul> | ||
<br> | <br> | ||
| - | <b>V09_10_2 Test digestion of Psb1c3 (including motA | + | <b>V09_10_2 Test digestion of Psb1c3 (including motA, motB or yhjH)</b><br> |
<ul> | <ul> | ||
| - | <li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | + | <li>Experiment: <br>Test digestion of Psb1c3 (including motA, yhjH or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> |
</ul> | </ul> | ||
<br> | <br> | ||
| - | <b>V09_10_3 Digestion of motA, motB and the promoters 20E, 20I and 18C</b><br> | + | <b>V09_10_3 Digestion of motA, motB, yhjH and the promoters 20E, 20I and 18C</b><br> |
<ul> | <ul> | ||
| - | <li>Experiment: <br> MotA | + | <li>Experiment: <br> MotA, MotB and yhjH were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).</li> |
</ul> | </ul> | ||
<br> | <br> | ||
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<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
<h2><b>V09_11 </b></h2><br> | <h2><b>V09_11 </b></h2><br> | ||
| - | <b>V09_11_1 Ligation and Transformation of motA, motB</b><br> | + | <b>V09_11_1 Ligation and Transformation of motA, motB and yhjH</b><br> |
<ul> | <ul> | ||
| - | <li>Experiment: <br> After the ligation of motA | + | <li>Experiment: <br> After the ligation of motA, motB and yhjH into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.</li> |
</ul> | </ul> | ||
<br> | <br> | ||
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<li>Experiment: <br> TEXT | <li>Experiment: <br> TEXT | ||
</ul> | </ul> | ||
| + | <br></td></tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
| + | <tr bordercolor="black" valign="top"> | ||
| + | <td width="900" bordercolor="black" valign="top"> | ||
| + | <h2><b>V09_12 </b></h2><br> | ||
| + | <b>V09_12_1 Quickchange of FliC part 2</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> Text</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <b>V09_12_2 Restriction and Ligation of promoter-RFP constructs into Psb1c3</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> Text</li> | ||
| + | </ul> | ||
| + | <br> | ||
<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
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