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Team:Goettingen/week20-2
From 2012.igem.org
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| - | <h2><b> | + | <h2><b>V09_10 </b></h2><br> |
| - | <b>Mini Prep of Psb1c3 (including motA or MotB)</b><br> | + | <b>V09_10_1 Mini Prep of Psb1c3 (including motA or MotB)</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li> | <li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li> | ||
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| - | + | <b>V09_10_2 Test digestion of Psb1c3 (including motA and motB)</b><br> | |
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<ul> | <ul> | ||
<li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | <li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | ||
</ul> | </ul> | ||
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| - | + | <b>V09_10_3 Digestion of motA, motB and the promoters 20E, 20I and 18C</b><br> | |
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<ul> | <ul> | ||
<li>Experiment: <br> MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | <li>Experiment: <br> MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | ||
</ul> | </ul> | ||
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| - | + | <b> V09_10_4 Quickchange of FliC</b><br> | |
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<ul> | <ul> | ||
<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed</li> | <li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed</li> | ||
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| - | <h2><b> | + | <h2><b>V09_11 </b></h2><br> |
| - | <b>Ligation and Transformation of motA, motB</b><br> | + | <b>V09_11_1 Ligation and Transformation of motA, motB</b><br> |
<ul> | <ul> | ||
| - | <li>Experiment: <br> After the ligation of motA and motB into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.</li> | + | <li>Experiment: <br> After the ligation of motA and motB into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.</li> |
</ul> | </ul> | ||
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Revision as of 11:58, 15 September 2012
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