Team:Goettingen/week9-2

From 2012.igem.org

(Difference between revisions)
Line 545: Line 545:
<li>Experiment:  <br>
<li>Experiment:  <br>
Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.The following protocol was considered.</li>
Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.The following protocol was considered.</li>
 +
</ul><br>
 +
 +
</td></tr>
 +
</table>
 +
 +
<br>
 +
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 +
<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
 +
<h2><b>V06_26 </b></h2><br>
 +
<b>V06_26_1 Preparation of chemically competent cells of the new E.coli (DH10B) strain</b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
The preparation of the competent cells was performed according to the following protocol. </li>
 +
<li>Observations & Results: <br>
 +
The performance of a test transformation proved the functionality of the new competent cells.
 +
</li></ul>
 +
<br>
 +
<b>V06_26_2 Preparation of over night cultures </b><br>
 +
<ul>
 +
<li>Experiment:  <br>
 +
In order to test different <i>E. coli</i> strains competent cells of the recently received strains BL21, DH5alpha and XL1 blue should be produced. On that account over night cultures of al strains were prepared. </li>
</ul><br>
</ul><br>

Revision as of 11:04, 15 September 2012