Team:Goettingen/week9-2

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Preparation of over night cultures

Experiment:
Since we received a new E. coli (DH10B) strain, over night cultures were prepared in order to produce new competent cells.
Observations & Results:

Preparation of over night cultures

Experiment:
Since we received a new E. coli (DH10B) strain, over night cultures were prepared in order to produce new competent cells.
Observations & Results:

V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into E. coli (DH10B)

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
Observations & Results:
The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

V06_19_2 Repetition of the chemical transformartion of the araC-promoter into E. coli (DH10B)

Experiment:
Since the last time the fact that the araC-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol.
Observations & Results:
The transformation was again not successful. Only the positive control showed any growth.

Preparation of over night cultures

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Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
+<h2><b>V06_25 </b></h2><br>
+<b>Preparation of over night cultures</i></b><br>
+<ul>
+<li>Experiment: <br>
+Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.<br>
+<li>Observations & Results: <br>
+</li>
+</table>
+<br>
 <table cellpadding="20 px" border="1" bordercolor="black" valign="top">
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