Team:Goettingen/week2-2

(Difference between revisions)

Revision as of 08:21, 14 September 2012

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Amplification of flhDC gene

Experiment:
Using the isolated genomic DNA of DH10B as template, the gene flhDC was amplified by Pfu-Polymerase according to the following protocol.

Separation of flhDC

Experiment:
The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
Observations & Results:
The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.

Purificaton of flhDC

Experiment:
The PCR-product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 538: / Line 538: @@
 <b>Amplification of <i>flhDC</i> gene</b><br>
 <ul>
-<li>Experiment: <br> Using the isolated genomic DNA of DH10B as template, the gene <i>flhDC</i> was amplified by Pfu-Polymerase according to the following protocol.</li>
+<li>Experiment: <br> Using the isolated genomic DNA of DH10B as template, the gene <i>flhDC</i> was amplified by Pfu-Polymerase according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#Competent_Cells">protocol</a>.</li>
 <br> </li>
 </ul>