Team:Goettingen/week4-2

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The transformation was successful. Numerous colonies on all plates as well as on the positive control. Now growth could be observed on the negative control plate, so the competent cells are preparation of the competent cells was correct this time. Additionally, no growth could be observed at the 14N plate. This is the only vector containing a kanamycin resistance instead of ampicillin. Eventually, the kanamycin concentration was too strong and inhibited growth.<br>
The transformation was successful. Numerous colonies on all plates as well as on the positive control. Now growth could be observed on the negative control plate, so the competent cells are preparation of the competent cells was correct this time. Additionally, no growth could be observed at the 14N plate. This is the only vector containing a kanamycin resistance instead of ampicillin. Eventually, the kanamycin concentration was too strong and inhibited growth.<br>
The strain (DH10B) grows very fast, thus try not to incubate too long to prevent formation of satellite colonies. <br>
The strain (DH10B) grows very fast, thus try not to incubate too long to prevent formation of satellite colonies. <br>
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The cells hosting plasmids with strong promoters are colored red due to the rfp gene fused to the promoter. This phenomenon can be used to verify correct transformation.
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The cells hosting plasmids with strong promoters are colored red due to the rfp gene fused to the promoter. This phenomenon can be used to verify correct transformation.<br> </li>
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<a href="#top">&uarr; Return to top</a>
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<b>Important pages</b>:<br>
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<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
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<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
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Revision as of 22:21, 13 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week4-2"