Team:Goettingen/week2-2

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Revision as of 20:43, 13 September 2012

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Amplification of flhDC gene

Experiment:
Using the isolated genomic DNA of DH10B as template, the gene flhDC was amplified by Pfu-Polymerase according to the following protocol.

Purificaton of flhDC

Experiment:
The amplicon was analsed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used. The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.

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Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 536: / Line 536: @@
 <td width="900" bordercolor="black" valign="top">
 <h2><b>V05_08 </b></h2><br>
-<b>Amplification of FlhDC via PCR</b><br>
+<b>Amplification of <i>flhDC</i> gene</b><br>
 <ul>
-<li>Experiment: <br> The genomic DNA of <i>E. coli</i> was isolated according to LINK!</li>
+<li>Experiment: <br> Using the isolated genomic DNA of DH10B as template, the gene <i>flhDC</i> was amplified by Pfu-Polymerase according to the following protocol.</li>
 <br> </li>
 </ul>
@@ Line 547: / Line 547: @@
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b>V05_11</b></h2><br>
+<h2><b>V05_10</b></h2><br>
-<b>Purificaton of FlhDC via Gel-extraktion kit</b></b></h2><br>
+<b>Purificaton of <i>flhDC</i> </b></b></h2><br>
 <ul>
-<li>Experiment:</li>
+<li>Experiment: <br>  The amplicon was analsed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used. The gel featured a clear band at about 900 kb. The amplification of <i>flhDC</i> was successful. </li>
 <br> </li>
 </ul>