Team:UC Chile2/General Protocols

From 2012.igem.org

(Difference between revisions)
Line 7: Line 7:
<h1>LB media</h1>
<h1>LB media</h1>
<ul>
<ul>
 +
<p>Final Concentrations</p>
<li>1% (w/v) tryptone</li>
<li>1% (w/v) tryptone</li>
<li>0.5% (w/v) yeast extract</li>
<li>0.5% (w/v) yeast extract</li>
Line 15: Line 16:
<li>5g NaCl</li>
<li>5g NaCl</li>
<br />
<br />
-
<li>15g Agar -Optional- /L</li>
+
<li>15g Agar -Optional-</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
</ul>
</ul>
<h1>SOB media</h1>
<h1>SOB media</h1>
<ul>
<ul>
 +
<p>Final Concentrations</p>
<li>0.5% (w/v) yeast extract</li>
<li>0.5% (w/v) yeast extract</li>
<li>2% (w/v) tryptone</li>
<li>2% (w/v) tryptone</li>
Line 41: Line 43:
<h1>5X Gibson Isothermal Buffer</h1>
<h1>5X Gibson Isothermal Buffer</h1>
<ul>
<ul>
 +
<p>Final Concentrations</p>
<li>25% PEG MW 8000</li>
<li>25% PEG MW 8000</li>
<li>500mM Tris HCl pH 7.5</li>
<li>500mM Tris HCl pH 7.5</li>
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<li>0.5g PEG MW 8000</li>
<li>0.5g PEG MW 8000</li>
<li>1.5mL Tris-HCl pH 7.5 1M</li>
<li>1.5mL Tris-HCl pH 7.5 1M</li>
-
<p>Swivel for 30 minutes in a orbital oscillator </p>
+
<p>Swivel for 30 minutes in a orbital oscillator for PEG to dissolve fully</p>
<li>50uL CaCl 2M</li>
<li>50uL CaCl 2M</li>
<li>100uL DTT 1M</li>
<li>100uL DTT 1M</li>

Revision as of 02:42, 12 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012