Team:UC Chile2/General Protocols

From 2012.igem.org

(Difference between revisions)
Line 11: Line 11:
<li>0.5% (w/v) yeast extract</li>
<li>0.5% (w/v) yeast extract</li>
<li>86 mM NaCl</li>
<li>86 mM NaCl</li>
-
<h2>Per liter:</h2>
+
<p>Per liter:</p>
<li>10g Tryptone</li>
<li>10g Tryptone</li>
<li>5g Yeast extract</li>
<li>5g Yeast extract</li>
<li>5g NaCl</li>
<li>5g NaCl</li>
 +
<br />
<li>15g Agar -Optional- /L</li>
<li>15g Agar -Optional- /L</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
Line 25: Line 26:
<li>2.5 mM KCl</li>
<li>2.5 mM KCl</li>
<li>20 mM MgSO4</li>
<li>20 mM MgSO4</li>
-
<h2>Per liter:</h2>
+
<p>Per liter:</p>
<li>5 g yeast extract</li>
<li>5 g yeast extract</li>
<li>20 g tryptone</li>
<li>20 g tryptone</li>
Line 31: Line 32:
<li>0.186 g KCl</li>
<li>0.186 g KCl</li>
<li>2.4 g MgSO4</li>
<li>2.4 g MgSO4</li>
 +
<br />
<li>15g Agar -Optional-</li>
<li>15g Agar -Optional-</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
<li>Adjust pH to 7.5 with NaOH 1M</li>
</ul>
</ul>

Revision as of 02:24, 12 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012