Team:Goettingen/week20-2
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- | <h2><b> | + | <h2><b>V09_10_4 </b></h2><br> |
<b>Quickchange of FliC</b><br> | <b>Quickchange of FliC</b><br> | ||
<ul> | <ul> | ||
- | <li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").</li> | + | <li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed</li> |
+ | </ul> | ||
+ | <br></td></tr> | ||
+ | </table> | ||
+ | <br> | ||
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+ | <h2><b>V09_11_1 </b></h2><br> | ||
+ | <b>Ligation of motA, motB into 20E, 20I and 18C followed by transformation into DH10B</b><br> | ||
+ | <ul> | ||
+ | <li>Experiment: <br> After the ligation of motA and motB into the plasmids with different promoters (20E, 20I and 18C) the ligation prodducts were transformed into [i]E. coli[/i] strain DH10B.</li> | ||
</ul> | </ul> | ||
<br></td></tr> | <br></td></tr> |
Revision as of 12:10, 11 September 2012
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