Team:Goettingen/week20-2

(Difference between revisions)

Revision as of 11:51, 11 September 2012

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Test digestion of Psb1c3 (including motA and motB)

Experiment:
Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones

Digestion of motA, motB and the promoters 20E, 20I and 18C

Experiment:
MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).

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Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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-<h2><b>V09_10 </b></h2><br>
+<h2><b>V09_10_1 </b></h2><br>
 <b>Test digestion of Psb1c3 (including motA and motB)</b><br>
 <ul>
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-<h2><b>V05_11</b></h2><br>
+<h2><b>V09_10_2 </b></h2><br>
-<b>Purificaton of FlhDC via Gel-extraktion kit</b></b></h2><br>
+<b>Digestion of motA, motB and the promoters 20E, 20I and 18C</b><br>
 <ul>
-<li>Experiment:</li>
+<li>Experiment: <br> MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).</li>
-<li>Description: according to the manual <br> </li>
-<li>Observations & Results: <br> </li>
 </ul>
 <br></td></tr>