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	<p><b>What we are planned to do:</b>		<p><b>What we are planned to do:</b>

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Revision as of 15:54, 9 September 2012

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Building a Toolkit

Part 1: The 96 direpeats

What we are planned to do:

We took the sequences of the four already known TAL Repeats A,C,G,T and combined them to 16 new, so called direpeat sequences. These 16 direpeats were ordered as gene synthesis products.
Now the real work began, to start building TAL Proteins we needed to expand our 16 direpeats a second time. Each of the 16 direpeats needed to be split into six versions, one version for every place of our final six direpeat respectevly twelve monorepeat TAL Protein. Because we didnt wanted to buy six times 16 different synthesised direpeats we came up with a plan to produce them by ourself. We created six primer pairs each primer with a common part matching everyone of the direpeats and an unique overhang to extend the direpeat it binds to.

After finshing these 96 different extension pcr's we had to ligate the products into the orignial igem biobrick vektor and finally got our full library of 96 unique direpeats