Team:HIT-Harbin

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<p><em>Staphylococcus aureus</em> infections are a major caus of morbidity and  mortality in community and hospital settings. Consequently, the emergence of  methicillin-resistant and, more recently, vancomysin-resistant strains of <em>S.aureus</em> represents an enormous threat to public health. Since bacterial sensors are  attracting more and more biologists' attention owing to its detecting  specifically, quickly and accurately, we plan to construct a <em>E.coli</em> consortium consisting of two different engineered populations, which comprises  consortium biofilm formation, <em>S.aureus </em>detecting, killing and bacterial  lysis devices, that will enable the <em>E.coli</em> consortium to detect and kill  a pathogen <em>S.aureus</em>.<br />
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  Firstly, a detecting system will be constructed. The engineered can detect the existence of <em>S.aureus </em>through sensing  the AIPs secreted only from <em>S.aureus</em>. The report will be shown  by the expression of GFP. Once it senses the AIPs, the engineered<em> E.coli</em> will release  signal protein AHL into the environment.<br />
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  Secondly, a killing and lysis system will be constructed. Upon sensing  the AHL, the engineered <em>E.coli </em>will express lysostaphin, an endopeptidase, which cleaves the pentaglycine cross-bridges of the staphylococal cell wall  rapidly lysing the bacteria. Some time after the lysostaphin is expressed, the  lysis E7 will be expressed and lysis <em>E.coli </em>so that  lysostaphin can be released into the environment.</p>
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Finally,  we will construct an enhanced consortium biofilm. The consortium biofilm is  composed of the two different engineered <em>E.coli</em> populations, which is  able to sense and kill <em>S.aureus</em>, respectively. The  enhanced consortium biofilm can promote intra- and inter-species signal  transduction, and stay stably. The detecting and killing system is distributed  in two different engineered <em>E.coli</em>. This will make functions  compartmental, and reduce metabolism load. On the other hand, this will promote  modularity in the level of bacteria
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<a class="logo" id="HIT-logo" href="HOME.html" title="HIT">Home</a>
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<li class="page_item page-item-14 "><a href="HOME.html" title="Home">Home</a></li>
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<li class="page_item page-item-39 current_page_item"><a href="TEAM.OVERVIEW.html" title="Alternate Home Page">TEAM</a>
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<li class="page_item page-item-136"><a href="TEAM.OVERVIEW.html" title="OVERVIEW">OVERVIEW</a>
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<li class="page_item page-item-136"><a href="TEAM.UNDERGRADUATES.html" title="UNDERGRADUATES">UNDERGRADUATES</a></li>
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<li class="page_item page-item-136"><a href="TEAM.FACILITATORS.html" title="FACILITATORS">FACILITATORS</a></li>
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<li class="page_item page-item-136"><a href="TEAM.ABOUT THE UNIVERSITY.html" title="ABOUT THE UNIVERSITY">ABOUT THE UNIVERSITY</a>
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<li class="page_item page-item-39 "><a href="PROJECT.OVERVIEW.html" title="PROJECT">PROJECT</a>
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<li class="page_item page-item-136"><a href="PROJECT.OVERVIEW.html" title="OVERVIEW">OVERVIEW</a></li>
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<li class="page_item page-item-136"><a href="PROJECT.PART 1.html" title="PART 1">PART 1</a></li>
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<li class="page_item page-item-136"><a href="PROJECT.PART 2.html" title="PART 2">PART 2</a></li>
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<li class="page_item page-item-136"><a href="PROJECT.PART 3.html" title="PART 3">PART 3</a></li>
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<li class="page_item page-item-136"><a href="PROJECT.Application.html" title="Application">Application</a>
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<li class="page_item page-item-39 c"><a href="PARTS.html" title="PARTS<">PARTS</a>
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<li class="page_item page-item-39 "><a href="SAFETY.html" title="SAFETY">SAFETY </a>
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<li class="page_item page-item-136"><a href="NOTEBOOK.DIARY.html" title="DIARY">DIARY</a></li>
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<li class="page_item page-item-136"><a href="NOTEBOOK.Attributions.html" title="Attributions<">Attributions</a></li>
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<li class="page_item page-item-39 "><a href="HUMAN PRACTICE.LECTURE" title="HUMAN PRACTICE">HUMAN PRACTICE</a>
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<li class="page_item page-item-136"><a href="HUMAN PRACTICE.LECTURE" title="LECTURE">LECTURE</a>
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<li class="page_item page-item-136"><a href="HUMAN PRACTICE.THE SOFTRWAR" title="THE SOFTRWARE">THE SOFTRWARE</a></li>
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<li class="page_item page-item-136"><a href="HUMAN PRACTICE.THE SONG" title="THE SONG">THE SONG</a></li>
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About us</div>
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<a>The HIT-Harbin team is composed of X motivated student across a wide range of academic backgrounds this year: from freshman to senior, from biology to electromechanics. The identical dream makes us together to share a passion for science, stay-up late in the lab and learn. We're currently taking great efforts to make it and enjoying the happiness and sadness it bring to us.</a>
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Revision as of 02:12, 9 September 2012


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