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Team:UC Chile2/Biosafety
From 2012.igem.org
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| - | Yes, the release of biobricks made from Synechocystis genome´s sequences or at least with a region homologous to it raises the possibility of recombination by environmental cyanobacteria. | + | Yes, the release of biobricks made from Synechocystis genome´s sequences or at least with a region homologous to it raises the possibility of recombination by environmental cyanobacteria. To address this issue we have proposed and designed a biosafety mechanism discussed in question number 4 (see below). |
| - | As DNA is always hermetically contained or discarded with other biological material according to standard protocols | + | Furthermore, our laboratory practices enforce strict methods to handle any biological material. As DNA is always hermetically contained or discarded with other biological material according to standard protocols we believe that our practices will not produce any environmental hazard. |
<ul> | <ul> | ||
<li><strong> did you document these issues in the Registry? </strong></li> | <li><strong> did you document these issues in the Registry? </strong></li> | ||
| - | It has to be stated that any of the sequences designed and/or handled by our team are per se dangerous nor they represent a fitness advantage to recombinant strains. Regarding to living cells, as they carry recombinant DNA, the same precautions as for that molecule apply. | + | It has to be stated that any of the sequences designed and/or handled by our team are not <i>per se</i> dangerous nor they represent a fitness advantage to recombinant strains. |
| + | |||
| + | <b>This is kinda strange</b> | ||
| + | Regarding to living cells, as they carry recombinant DNA, the same precautions as for that molecule apply. | ||
<li><strong> how did you manage to handle the safety issue? </strong> </li> | <li><strong> how did you manage to handle the safety issue? </strong> </li> | ||
| - | Recombinant and non-recombinant cells are exposed to a heat shock ( | + | <b>Yo creo que acá no están hablando de como descartar las células...</b> |
| + | Recombinant and non-recombinant cells are exposed to a heat shock (95°) previous to ethanol washing and discard in biosafety cabinets | ||
Our lab followed the Manual of Biosafety, established by the Superior Counsel of Science and Technological Development, from the National Fund of Scientific and Technological Development of Chile (FONDECYT).(1) | Our lab followed the Manual of Biosafety, established by the Superior Counsel of Science and Technological Development, from the National Fund of Scientific and Technological Development of Chile (FONDECYT).(1) | ||
<li><strong> How could other teams learn from your experience? </strong> </li> | <li><strong> How could other teams learn from your experience? </strong> </li> | ||
| - | For adressing the possibility of recombinant Synechocystis cells being released from the lab, we designed a recombination plasmid that knocks out the copS gene. This gene codes for a Cu-binding protein and is essential to Cu stress response in Synechocystis. It has been | + | For adressing the possibility of recombinant Synechocystis cells being released from the lab, we've designed a recombination plasmid that knocks out the copS gene. This gene codes for a Cu-binding protein and is essential to Cu stress response in Synechocystis. It has been demonstrated that strains lacking this gene can´t survive in much lesser Cu concentrations found in drink water or natural water bodies (2). |
| - | We advice | + | |
| + | We advice every team working in this chassis to adopt similar strategies. By designing integrative plasmids which interrupt fitness related genes, recombinant strains will be auxotrophic and/or hiper-suceptible. Thus, their recombinant cyanobacterial strains will be unable to thrive in natural environments. | ||
</ul> | </ul> | ||
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<li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong> </li> | <li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong> </li> | ||
| - | Yes, in our institution we have a local biosafety panel. The Comitee of Bioethics and Biosafety of the Faculty of Biological Sciences of the Pontifical Catholic University of Chile has reviewed our work | + | Yes, in our institution we have a local biosafety panel. The Comitee of Bioethics and Biosafety of the Faculty of Biological Sciences of the Pontifical Catholic University of Chile has reviewed our work and determined that the procedures involved in our project follow the bioethical and biosafety standards for research regulations provided by the Chilean Comission on Scientific and Technological Research (CONICYT). |
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Revision as of 19:50, 7 September 2012
- Would any of your project ideas raise safety issues in terms of:
- researcher or public safety,
- environmental safety?
No pathogenic microorganisms or dangerous genes were part of our ideas, in consequence, our intended projects, if executed, do not represent any risk to researchers nor public safety.
As synechocystis is a naturally competent environmental bacteria that undergoes homologous recombination, the idea of genetically engineer it raises environmental safety issues regarding the acquisition of biobricks by other cyanobacteria outside the lab.
- Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
Yes, the release of biobricks made from Synechocystis genome´s sequences or at least with a region homologous to it raises the possibility of recombination by environmental cyanobacteria. To address this issue we have proposed and designed a biosafety mechanism discussed in question number 4 (see below).
Furthermore, our laboratory practices enforce strict methods to handle any biological material. As DNA is always hermetically contained or discarded with other biological material according to standard protocols we believe that our practices will not produce any environmental hazard.
- did you document these issues in the Registry?
- how did you manage to handle the safety issue?
- How could other teams learn from your experience?
It has to be stated that any of the sequences designed and/or handled by our team are not per se dangerous nor they represent a fitness advantage to recombinant strains.
This is kinda strange Regarding to living cells, as they carry recombinant DNA, the same precautions as for that molecule apply.
Yo creo que acá no están hablando de como descartar las células... Recombinant and non-recombinant cells are exposed to a heat shock (95°) previous to ethanol washing and discard in biosafety cabinets Our lab followed the Manual of Biosafety, established by the Superior Counsel of Science and Technological Development, from the National Fund of Scientific and Technological Development of Chile (FONDECYT).(1)
For adressing the possibility of recombinant Synechocystis cells being released from the lab, we've designed a recombination plasmid that knocks out the copS gene. This gene codes for a Cu-binding protein and is essential to Cu stress response in Synechocystis. It has been demonstrated that strains lacking this gene can´t survive in much lesser Cu concentrations found in drink water or natural water bodies (2).
We advice every team working in this chassis to adopt similar strategies. By designing integrative plasmids which interrupt fitness related genes, recombinant strains will be auxotrophic and/or hiper-suceptible. Thus, their recombinant cyanobacterial strains will be unable to thrive in natural environments.
- Is there a local biosafety group, committee, or review board at your institution?
- Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
Yes, in our institution we have a local biosafety panel. The Comitee of Bioethics and Biosafety of the Faculty of Biological Sciences of the Pontifical Catholic University of Chile has reviewed our work and determined that the procedures involved in our project follow the bioethical and biosafety standards for research regulations provided by the Chilean Comission on Scientific and Technological Research (CONICYT).
Click here to download our Certificate from the Comitee of Bioethics and Biosafety
Our lab follows the Manual of Biosafety, established by the Superior Counsel of Science and Technological Development, from the National Fund of Scientific and Technological Development of Chile (FONDECYT).
Click here to download the Manual of Biosafety
To prevent future problems and to encourage the construction of biosafe standard biological parts, we, UC_Chile team, propose the creation of the section “biosafety analysis” mandatory for all parts before their shipping to the registry. In this way, is assured that each new biobrick or device includes an assessment of the risks and biosafety advantages involved in its physical implementation.
Alongside the previous, we suggest the characterization of biosafety levels of other team’s constructs, making this practice analogous to the existing standard characterization of bricks. The requirement of this biosafety assessment for a team to win the gold medal would be a strong incentive.
Regarding to the molecular methods by which biosafety could be improved; we believe that the addition of a “bomb” gene to all iGEM plasmid backbones could be very useful. This gene would kill the recombinant cells unless its action is inhibited by a molecule present in the lab culture media but with an insignificant concentration in the environment.
We have selected a gen that placed under a constitutive promoter would destroy the cells. This gen is not available in the Registry, and soon we will document more information about it in our wiki.
