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Team:Freiburg/Project/Overview
From 2012.igem.org
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| - | We took the sequences of the four already known TAL Repeats A,C,G,T and combined them to 16 new, so called direpeat sequences. These 16 direpeats were ordered as gene synthesis products. <BR>Now the real work began, to start building TAL Proteins we needed to expand our 16 direpeats a second time. Each of the 16 direpeats needed to be split into six versions, one version for every place of our final six direpeat respectevly twelve monorepeat TAL Protein. Because we didnt wanted to buy six times 16 different synthesised direpeats we came up with a plan to produce them by ourself. We created six primer pairs each primer with a common part matching everyone of the direpeats and an unique overhang to extend the direpeat it binds to. | + | We took the sequences of the four already known TAL Repeats A,C,G,T and combined them to 16 new, so called direpeat sequences. These 16 direpeats were ordered as gene synthesis products. <BR>Now the real work began, to start building TAL Proteins we needed to expand our 16 direpeats a second time. Each of the 16 direpeats needed to be split into six versions, one version for every place of our final six direpeat respectevly twelve monorepeat TAL Protein. Because we didnt wanted to buy six times 16 different synthesised direpeats we came up with a plan to produce them by ourself. We created six primer pairs each primer with a common part matching everyone of the direpeats and an unique overhang to extend the direpeat it binds to. |
Revision as of 13:10, 7 September 2012
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Overview
What we are planned to do:
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We took the sequences of the four already known TAL Repeats A,C,G,T and combined them to 16 new, so called direpeat sequences. These 16 direpeats were ordered as gene synthesis products.
Now the real work began, to start building TAL Proteins we needed to expand our 16 direpeats a second time. Each of the 16 direpeats needed to be split into six versions, one version for every place of our final six direpeat respectevly twelve monorepeat TAL Protein. Because we didnt wanted to buy six times 16 different synthesised direpeats we came up with a plan to produce them by ourself. We created six primer pairs each primer with a common part matching everyone of the direpeats and an unique overhang to extend the direpeat it binds to.
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After finshing these 96 different direpeats we had to ligate them into the orignial igem biobrick vektor and start experimenting.
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