User talk:Jarlemag

From 2012.igem.org

Contents

Tasks

  • Make cloramphenicol stock. Check.
  • Make glycerol stocks of important parts. Discard old plates. Check.
  • Perform CFU-assay of lysis induced cells. Check. Redo?
  • Combine LuxR-HSL and lysis device.
  • Combine pBAD and GFP
  • Research lactate production in E. coli
  • Write more for the book chapter
  • Research QuikChange


Useful links

http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000

http://www.troemner.com/pdf/PipetteStandardsHandbook.pdf'

http://www.pipette.com/Support/GuideToPipetting.pdf


http://2008.igem.org/Team:EPF-Lausanne/2-step_PCR

http://2009.igem.org/Team:UNIPV-Pavia/Parts_Characterization#Microplate_reader_experiments

Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits

Structural Biology Target Registration Database

DNASU plasmid repository

Addgene plasmid repository

PlasmID database


Moss lab protocols

Lactate genes and promoters:

Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate

Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production†

KEGG: cg1027

[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR

Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif

Promoters of Corynebacterium glutamicum

C. glutamicum lldR crystal structure


RNA polymerase:

Oriented, active Escherichia coli RNA polymerase: an atomic force microscope study

Sequence Specific Interaction between Promoter DNA and Escherichia coli RNA Polymerase: Comparative Thermodynamic Analysis with One Immobilized Partner

Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase

Wang et al, 1995: Discontinuous movements of DNA and RNA in RNA polymerase accompany formation of a paused transcription complex

DNA oligo and gene synthesis

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Product-Types/Primers-Oligos-Nucleotides/invitrogen-custom-dna-oligos/Technical-Resources-for-Oligonucleotides/DNA-Oligo-FAQ.html#1

PCR

PCR Principles and Practices

New England Biolabs: PCR Guidelines

http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm

Premier biosoft: PCR primer design guidelines

http://www.cybertory.org/exercises/primerDesign/index.html

http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

10 tips for designing PCR primers that work

How to make oligonucleotide primers for PCR


Software

http://molbiol-tools.ca/molecular_biology_freeware.htm


quorum sensing

Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99

N-acyl-L-homoserine lactone signal interception by Escherichia coli.


Other

Syntetisk biologi: Genmodifisert bakterie drep annan bakterie

http://en.wikipedia.org/wiki/DNA_ligase