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From 2012.igem.org

13 Split GFP experiments

 

13.1 Co-transformation:

13.1.1 Mix the plasmids of D0, FA and FB.

13.1.2 Transform the mixed plasmids to BL21*(DE3)

 

13.2 Pick a colony from the plate Co-transformation. Grow in LB(50 μg/mL Ampicillin, 25 μg/mL Spectinomycin, 25 μg/mL Kanamycin)

 

13.3 Induce the cells by 0.2M IPTG for 2 hours at mid-log phase.

 

13.4 Wash the cells twice with equivalent PBS

 

13.5 Test with Synergy H1 Hybrid reader

 

13.6 Take picture with Confocal Scanning Microscope.