Team:ZJU-China/n p 13.htm



13 Split GFP experiments


13.1 Co-transformation:

13.1.1 Mix the plasmids of D0, FA and FB.

13.1.2 Transform the mixed plasmids to BL21*(DE3)


13.2 Pick a colony from the plate Co-transformation. Grow in LB(50 μg/mL Ampicillin, 25 μg/mL Spectinomycin, 25 μg/mL Kanamycin)


13.3 Induce the cells by 0.2M IPTG for 2 hours at mid-log phase.


13.4 Wash the cells twice with equivalent PBS


13.5 Test with Synergy H1 Hybrid reader


13.6 Take picture with Confocal Scanning Microscope.