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From 2012.igem.org

Home Team Official Team Profile Project Parts Submitted Modeling Notebook Attributions

Contents 1 Notebook for week of July 30 to August 5 1.1 Design of Converter Plasmid 1.2 Design of Reporter Plasmid 1.3 Spinach Aptamer 1.4 CBB5 Caffeine Inducible Promoters 1.5 Inducible Odor

Notebook for week of July 30 to August 5

Design of Converter Plasmid

• Razan
  • 7/31- Sequenced DNA prepped from gibson transformation colonies- plasmids had errors in sequence.
  • 8/2- Redid gibson assembly reactions and re-transformed using filter before transformation.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

• Peter
  • 7/31- Retry of cotransformation of PRA301-ndmApro LacZ reporter plasmid with orf1- 4,5,6 mini-preps. Used new miniprep from Ben, only 0.5 ul of each plasmid per transformation. 3 cotransformations, 4 single transformation for each plasmid.
  • 8/1- 2 colonies picked from each cotransformation plate.
  • 8/2- Glycerol stock saved of 1st colony from 1st cotransformation plate (ORF1-4 + ndmApro)
  • 8/3- Transformation of ORF1-4 plasmid into TOP10 cells

Inducible Odor