User:David.lim.yale/18 July 2012
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DrJones1935 (Talk | contribs) (Created page with "====Mini Prep the two O/N cultures with growth==== *Follow Mini Prep protocol outlined here:Spencer_Katz_Lab_Notebook#III._Miniprep_.28QIAGEN.29_Plasmid_Cultures *''Note: Acc...") |
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====Mini Prep the two O/N cultures with growth==== | ====Mini Prep the two O/N cultures with growth==== | ||
- | *Follow Mini Prep protocol | + | *Follow Mini Prep protocol |
*''Note: Accidentally used 500 μL EB buffer to elute DNA instead of 50 μL. Depending on the Nano Drop concentration reading, may use EtOH/PCA extraction to concentrate DNA to obtain a usable concentration. In the meantime, the procedure will be repeated.'' | *''Note: Accidentally used 500 μL EB buffer to elute DNA instead of 50 μL. Depending on the Nano Drop concentration reading, may use EtOH/PCA extraction to concentrate DNA to obtain a usable concentration. In the meantime, the procedure will be repeated.'' |
Latest revision as of 03:54, 4 October 2012
Mini Prep the two O/N cultures with growth
- Follow Mini Prep protocol
- Note: Accidentally used 500 μL EB buffer to elute DNA instead of 50 μL. Depending on the Nano Drop concentration reading, may use EtOH/PCA extraction to concentrate DNA to obtain a usable concentration. In the meantime, the procedure will be repeated.