User contributions
From 2012.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 17:06, 26 October 2012 (diff | hist) Team:Yale/Project (top)
- 16:49, 26 October 2012 (diff | hist) N File:Replication fork.png (Yale 2012) (top)
- 16:43, 26 October 2012 (diff | hist) Team:Yale/Project
- 04:04, 4 October 2012 (diff | hist) m Team:Yale/Project (→Generating a beta protein library)
- 04:03, 4 October 2012 (diff | hist) Team:Yale/Project (→Generating a beta protein library)
- 04:02, 4 October 2012 (diff | hist) Team:Yale/Project
- 03:58, 4 October 2012 (diff | hist) N User:David.lim.yale/1 August 2012 (Created page with "====Mini Prep O/N culture of pBAV1K==== *Final concentration of 86.7 ng/μL *Add 19.25 μL of Elution Buffer to bring concentration to 80ng/μL ====Observe the results for the t...") (top)
- 03:57, 4 October 2012 (diff | hist) N User:David.lim.yale/31 July 2012 (Created page with "====Transform Ac, Bs, Ec strains as above (24 July)==== *''Note: The concentration of <span style="color:#00CC00">Kan</span> was increased to 50 μg/mL for Ec and Ac, and kept at...") (top)
- 03:57, 4 October 2012 (diff | hist) N User:David.lim.yale/30 July 2012 (Created page with "====Make plates of pBAV1K-containing DH5α glycerol stocks==== *Inoculate a sample of frozen DH5α cells containing the pBAV1K-T5-''gfp'' plasmid onto LB-<span style="color:#00CC...") (top)
- 03:56, 4 October 2012 (diff | hist) N User:David.lim.yale/24 July 2012 (Created page with "====Transform WT ADP1 using natural competence==== *Inoculate 20 μL of the O/N cultures from yesterday into 300 μL of the appropriate medium (LB or SOB), with two tubes for eac...") (top)
- 03:56, 4 October 2012 (diff | hist) N User:David.lim.yale/23 July 2012 (Created page with "====Make Super Optimal Broth (SOB) medium==== *Add 20 (20.0) g Bacto-tryptone, 5 (5.0) g yeast extract, 0.584 (0.5849) g NaCl for 10 mM, and 0.186 (0.1864) g KCl for 2.5 mM *Brin...") (top)
- 03:55, 4 October 2012 (diff | hist) N User:David.lim.yale/21 July 2012 (Created page with "====Make glycerol stocks (3x) of pBR322-transformed XL1-Blue O/N cultures==== *Aliquot 600 μL of culture from successful tube (1 of 2) into 3 Eppendorf tubes *Add 200 μL to eac...") (top)
- 03:55, 4 October 2012 (diff | hist) N User:David.lim.yale/20 July 2012 (Created page with "====Prepare Bs competent cells from strains of interest==== *Prepare fresh SpC medium (4x 20 mL in 125-mL E. flasks) by adding the following to 40 mL 5X Spizizen salts solution i...") (top)
- 03:54, 4 October 2012 (diff | hist) N User:David.lim.yale/19 July 2012 (Created page with "====Make O/N cultures (3x) of pBR322 Ec XL1-Blue competent cells==== *''Performed as on Tues, July 17'' *A plate was also inoculated from a robust colony to obtain a new fresh la...") (top)
- 03:54, 4 October 2012 (diff | hist) User:David.lim.yale/18 July 2012 (top)
- 03:53, 4 October 2012 (diff | hist) N User:David.lim.yale/18 July 2012 (Created page with "====Mini Prep the two O/N cultures with growth==== *Follow Mini Prep protocol outlined here:Spencer_Katz_Lab_Notebook#III._Miniprep_.28QIAGEN.29_Plasmid_Cultures *''Note: Acc...")
- 03:53, 4 October 2012 (diff | hist) N User:David.lim.yale/17 July 2012 (Created page with "====Make glycerol stocks of Pedraza-Reyes strains==== *Aliquot 600 mL of the O/N cultures of PERM311, PERM704, and PERM739 into Eppendorf tubes *Add 200 mL 60% glycerol solution ...") (top)
- 03:52, 4 October 2012 (diff | hist) N User:David.lim.yale/16 July 2012 (Created page with "====Prepare plates of Bs strains to make competent==== *As per the two-step transformation procedure in the "Protocols" page, plate the following strains as lawns: **Bs1A833 (''...") (top)
- 03:52, 4 October 2012 (diff | hist) N User:David.lim.yale/13 July 2012 (Created page with "====Make selection plates for <span style="color:#FF3399">Neo</span> (20 μg/mL)==== *Bring 6.25 (6.26) g LB powder and 3.75 (3.74) g agar to 250 mL with dH<sub>2</sub>O in an Er...") (top)
- 03:51, 4 October 2012 (diff | hist) N User:David.lim.yale/12 July 2012 (Created page with "====Re-plate plasmid-containing cultures==== *The Ec DH5α cells containing the plasmids pBAV1K-T5 and pIM1463 were re-plated from the original plates made (7/09/12)to obtain fre...") (top)
- 03:51, 4 October 2012 (diff | hist) N User:David.lim.yale/9 July 2012 (Created page with "====Make selection plates for <span style="color:#00CC00">Kan</span> and <span style="color:#CC3300">Tet</span>==== *Prepare LB agar as per usual *'''For <span style="color:#00CC...") (top)
- 03:50, 4 October 2012 (diff | hist) N User:David.lim.yale/8 July 2012 (Created page with "====Streak Bs strains 168 and PY79 on fresh plates for single colonies==== *Observations on the plates prepared from the filter disks: **1A1 (168): **1A7474 (PY79): *Pick a singl...") (top)
- 03:50, 4 October 2012 (diff | hist) N User:David.lim.yale/6 July 2012 (Created page with "====Inoculate plate culture for Bs strain 1A1 and 1A747 received today==== *Place spore-ridden filter disks (3x) of Bs strains 1A1 (168) and 1A747 (PY79) from BGSC on LB plates *...") (top)
- 03:49, 4 October 2012 (diff | hist) N User:David.lim.yale/5 July 2012 (Created page with "====Make 10X phosphate buffered saline (PBS) stock solution==== *For 1 L of 10X PBS (pH ~6.8 before dilution to 1X), mix: **80 g (80.06) NaCl **2 g (1.99735) KCl **14.4 g (14.423...") (top)
- 03:49, 4 October 2012 (diff | hist) N User:David.lim.yale/3 July 2012 (Created page with "===Make LB plate cultures from glycerol stocks==== *Glycerol stocks from the following strains were taken one-by-one from the -80<sup>o</sup>C freezer and put on ice for plating:...") (top)
- 03:48, 4 October 2012 (diff | hist) N User:David.lim.yale/27 June 2012 (Created page with "====Make 34 mg/mL <span style="color:#0000CC">Chloramphenicol</span> stock solution for plates/liquid cultures==== *Weigh out 340 mg 339.72 mg of <span style="color:#0000CC">Chl...") (top)
- 03:48, 4 October 2012 (diff | hist) N User:David.lim.yale/22 June 2012 (Created page with "====Make 15% glycerol stocks (3 total) of successful O/N culture of Bs strain 1A833==== *Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3) *Add 200 uL of ...") (top)
- 03:47, 4 October 2012 (diff | hist) N User:David.lim.yale/21 June 2012 (Created page with "====Inoculate O/N cultures for successful plate of Bs strain 1A833==== *Inoculate one isolated colony (or as close as possible) into ~2 mL of LB+<span style="color:#660066">Spc<...") (top)
- 03:47, 4 October 2012 (diff | hist) N User:David.lim.yale/20 June 2012 (Created page with "====Make selection plates for <span style="color:#660066">Spc</span>, <span style="color:#FF0000">Amp</span>, <span style="color:#00CC00">Kan</span>==== *Weigh out 25 g of LB (M...") (top)
- 03:46, 4 October 2012 (diff | hist) N User:David.lim.yale/18 June 2012 (Created page with "====Make 10 mg/ mL <span style="color:#660066">Spc</span> stock solution for plates/liquid cultures==== *Weigh out 250 mg 257.92 mg of <span style="color:#660066">Spc</span> pow...") (top)
- 03:46, 4 October 2012 (diff | hist) N User:David.lim.yale/6 June 2012 (Created page with "====Make nutrient broth (1 L) for liquid cultures==== *Weigh out and combine 5 g of yeast extract and 10 g of peptone *Bring to 900 mL with dH<sub>2</sub>O water and allow solid...") (top)
- 03:44, 4 October 2012 (diff | hist) Team:Yale/Project (→David's write-up)
- 03:43, 4 October 2012 (diff | hist) N File:PBAV1K-T5-gfp plasmid map.png (top)
- 03:40, 4 October 2012 (diff | hist) Team:Yale/Project (→Implementation of the MAGE methodology through continuous cycles of recombination)
- 03:40, 4 October 2012 (diff | hist) Team:Yale/Project (→Isolation of ssDNA-binding recombinase proteins that bind mutagenic oligos)
- 03:39, 4 October 2012 (diff | hist) Team:Yale/Project (→Experimental Methodology)
- 03:34, 4 October 2012 (diff | hist) Team:Yale/Project (→Crazy Ideas to Think About)
- 03:33, 4 October 2012 (diff | hist) Team:Yale/Project (→cT5 (normal))
- 03:31, 4 October 2012 (diff | hist) N File:Yale cassette map.png (top)
- 03:27, 4 October 2012 (diff | hist) Team:Yale/Project (→cT5 (normal))
- 03:13, 4 October 2012 (diff | hist) User:DrJones1935/30 September 2012 (→RESULTS:) (top)
- 03:06, 4 October 2012 (diff | hist) N File:Spencer Notebook 11.jpg (top)
- 03:06, 4 October 2012 (diff | hist) N File:Spencer Notebook 10.jpg (top)
- 03:05, 4 October 2012 (diff | hist) N File:Spencer Notebook 9.jpg (top)
- 03:00, 4 October 2012 (diff | hist) Team:Yale/Project (→Yeast Assembly)
- 02:50, 4 October 2012 (diff | hist) N File:Spencer Notebook 8.jpg (top)
- 02:49, 4 October 2012 (diff | hist) N File:Spencer Notebook 7.jpg (top)
- 02:48, 4 October 2012 (diff | hist) N File:Spencer Notebook 6.jpg (top)
- 02:46, 4 October 2012 (diff | hist) N File:Spencer Notebook 5.jpg (top)
- 02:46, 4 October 2012 (diff | hist) N File:Spencer Notebook 4.jpg (top)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)