User:David.lim.yale/18 July 2012

From 2012.igem.org

Mini Prep the two O/N cultures with growth

  • Follow Mini Prep protocol
  • Note: Accidentally used 500 μL EB buffer to elute DNA instead of 50 μL. Depending on the Nano Drop concentration reading, may use EtOH/PCA extraction to concentrate DNA to obtain a usable concentration. In the meantime, the procedure will be repeated.