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Team:XMU-China
From 2012.igem.org
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| + | <p>E.Lumoli: a shining synthetic device for digit or time-course display.<br> | ||
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| + | <p> XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device… | ||
<hr><a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[see more]</a></div> | <hr><a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[see more]</a></div> | ||
Revision as of 07:22, 12 September 2012
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E.Lumoli: a shining synthetic device for digit or time-course display. XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device… [see more] Sunday, August 26th
Digital Display: Ligated 18AI and RETPcPb and put the ligation product in 16ºC overnight for the transformation in the next day. Time Delay: Goal: To do the ligation of pBAD-RBS0.6 and pBAD-RBS0.07. Isolate plasmids and ran on a gel. The result for analysis was correct. Cause the bands of pBAD hardly to be seem, we increased the volume of pBAD when ligation. Fluorescence Test: We changed the abrabinose concentration from high levels to lower ones according to last fluorescence measurements of PBADRLT. Besides, we took some kinetic curves in all concentration levels we had taken before for comparison. The analysis of these data will help us improve our efficiency in the coming fluorescence measurements Design: We did some card design and drew some drafts. [see more] |
DEVICE DESCRIPTION E.LUMOLI DISPLAYER
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| Digital Display | Immobilization | Time delay |
| Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently. | Immobilization of Fluorescent E.coli cells .Design A Bioreactor for Numeric Display | Testing the delay of time for GFP expression when we use different strength of RBS |
