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Team:University College London/LabBook/Week5
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| - | == | + | == Monday (9.7.12) == |
| + | |||
| + | '''Aim: Transformation of Key BioBricks''' | ||
| + | |||
| + | '''Method''' | ||
| + | |||
| + | (LOGO) Transformation Protocol 2 | ||
| + | |||
| + | '''Step 1 – Thawing Cells:''' Use W3100 cell line created in Week 2 (Expt 2.1) | ||
| + | '''Step 3 – Addition of BioBrick:''' To each 2ml eppendorf, add 1ul of the following BioBricks. Include an extra tube as a control, with no BioBrick added. | ||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! colspan="2" | !! Function !! Module | ||
| + | |- | ||
| + | | rowspan="8" |BioBrick|| BBa_K123003 || Oestrogen Receptor || Detection | ||
| + | |- | ||
| + | | BBa_K123002 || Oestrogen Response Element|| Detection | ||
| + | |- | ||
| + | | BBa_K398108 || Salt Tolerance Cluster || Salt Tolerance | ||
| + | |- | ||
| + | | BBa_J23100|| Constitutive Promoter || | ||
| + | |- | ||
| + | |BBa_J23107|| Constitutive Promoter || | ||
| + | |- | ||
| + | | BBa_R0040|| TetR Repressible Promoter || Buoyancy | ||
| + | |- | ||
| + | |BBa_J23106|| Constitutive Promoter || | ||
| + | |- | ||
| + | |BBa_B0034|| Ribosome Binding Site (RBS)|| All | ||
| + | |- | ||
| + | | Control || (No BioBricks)|| || | ||
| + | |} | ||
| + | |||
| + | '''Step 9 – Plating samples on Agar Plates:''' The table below indicates the chosen inoculation volume (two for each BioBrick) and the correct gel antibiotic concentration for all samples. | ||
| + | |||
| + | |||
| + | |||
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== 4.1 == | == 4.1 == | ||
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Revision as of 11:38, 1 August 2012
Contents |
Monday (9.7.12)
Aim: Transformation of Key BioBricks
Method
(LOGO) Transformation Protocol 2
Step 1 – Thawing Cells: Use W3100 cell line created in Week 2 (Expt 2.1) Step 3 – Addition of BioBrick: To each 2ml eppendorf, add 1ul of the following BioBricks. Include an extra tube as a control, with no BioBrick added.
| Function | Module | ||
|---|---|---|---|
| BioBrick | BBa_K123003 | Oestrogen Receptor | Detection |
| BBa_K123002 | Oestrogen Response Element | Detection | |
| BBa_K398108 | Salt Tolerance Cluster | Salt Tolerance | |
| BBa_J23100 | Constitutive Promoter | ||
| BBa_J23107 | Constitutive Promoter | ||
| BBa_R0040 | TetR Repressible Promoter | Buoyancy | |
| BBa_J23106 | Constitutive Promoter | ||
| BBa_B0034 | Ribosome Binding Site (RBS) | All | |
| Control | (No BioBricks) | ||
Step 9 – Plating samples on Agar Plates: The table below indicates the chosen inoculation volume (two for each BioBrick) and the correct gel antibiotic concentration for all samples.
4.1
5.3
5.2
