Team:UT-Tokyo/Project/H2 E.coli/Results

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== Result ==
== Result ==
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We measured the amount of H₂ produced by each E.coli transducted “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) with wild type by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]].<br />
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We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page “Assay”. In this page, we describe the data.
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In this page, we describe the date.
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The result is  the following (Fig.1)
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The result is as the following graph(Fig.1)
 
[[file:UT Tokyo Fig diagram 640 480.png]]
[[file:UT Tokyo Fig diagram 640 480.png]]
   
   
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Fig.1:H₂production in liquid LB medium of each glucose concentration.<br />
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Fig.1:H₂production in liquid LB medium containing the labeled glucose concentration.
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This vertical axis expresses v/v% of H₂ per the gaseous phase after we cultivate each E.coli for 8 hour in the situation the liquid LB medium and the gaseous phase is 1mL.And this horizontal one shows that the kinds of E.coli and the concentrations of glucose of each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.<br />
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The vertical axis expresses v/v% of H₂ in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.
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The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br />
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*The amount of H₂ production is fhlA>fhlA(E363G)>wild type in the same situations.<br />
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The results shown by the Fig.1 is statistically sufficient to conclude the followings.
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*There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration of the liquid LB concentration.
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1. The amount of H₂ production is fhlA>fhlA(E363G)>wild type in these conditions.
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2. There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration in the liquid LB.
   
   
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The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]].
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The detailed interpretations of this result are done in the “Discussion”.[[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]].

Revision as of 22:41, 26 September 2012

H2 E.coli: Results

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このページの概要を、簡単に記述。

Result

We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page “Assay”. In this page, we describe the data. 

The result is  the following (Fig.1)


UT Tokyo Fig diagram 640 480.png

Fig.1:H₂production in liquid LB medium containing the labeled glucose concentration. The vertical axis expresses v/v% of H₂ in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.

The results shown by the Fig.1 is statistically sufficient to conclude the followings. 1. The amount of H₂ production is fhlA>fhlA(E363G)>wild type in these conditions. 2. There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration in the liquid LB. 

The detailed interpretations of this result are done in the “Discussion”.Discussion.



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