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Team:UIUC-Illinois/Notebook/Protocols/Inoculation
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| - | <center><h1> | + | <center><h1>Inoculation</h1></center><br/> |
| + | What you need:<br/> | ||
| + | - Falcon tubes<br/> | ||
| + | - The appropriate liquid media<br/> | ||
| + | - The appropriate antibiotic<br/> | ||
| + | - An agar plate containing the cells of interest or a frozen stock solution<br/> | ||
| + | - Autoclaved pipet tips<br/><br/> | ||
| + | Procedure: <br/><br/> | ||
| + | 1. To an autoclaved glass test tube with lid, add: 2-5 mL liquid media, 2-5 ul antibiotic (dilute to 1/1000)<br/> | ||
| + | 2. Using a pipet tip, gently poke a single colony of plated cells, or scratch cells onto the stick form the frozen freezer stock (taking colonies from a plate typically yields better results)<br/> | ||
| + | 3. Stir cells into the liquid media<br/> | ||
| + | 4. Label the test tubes and place them in 37˚C warm room overnight to grow (12-16 hrs)<br/><br/> | ||
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</div> | </div> | ||
Latest revision as of 15:07, 4 June 2012
