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Team:UIUC-Illinois/Notebook/MeetingNotes
From 2012.igem.org
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<center><h2>May-July</h2></center> | <center><h2>May-July</h2></center> | ||
| - | <li><a name="meet19">5/25/12</a></li> | + | <li><a name="meet18.5">5/1/12</a></li> |
| + | <li><a name="meet19" >5/25/12</a></li> | ||
<li><a name="meet20" >5/29/12</a></li> | <li><a name="meet20" >5/29/12</a></li> | ||
<li><a name="meet21" >6/1/12</a></li> | <li><a name="meet21" >6/1/12</a></li> | ||
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reading day’s social outing.</textarea> | reading day’s social outing.</textarea> | ||
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| + | <div id="meet18.5" style="display:none"> | ||
| + | <textarea readnoly rows="30" wrap="soft" | ||
| + | iGEM Advisors Meeting | ||
| + | 5/1/12 | ||
| + | |||
| + | Announcements | ||
| + | Courtney: $500 is needed for travel funds because of a new fee that requires $25 per ticket, in addition to the ticket price. We will continue looking at departments and companies for funding, but all meetings to request funding should be set up with Courtney present. We should also contact companies in research park (Adi will take the lead on this). Our goal is to recruit $10,000 from various departments and companies in order to be financially secure this year and set up next year’s team. Adi will contact the ACES department (through Bhalerao) and Steven Sliger of MCB. | ||
| + | |||
| + | Agenda | ||
| + | Wetlab Summer Plans | ||
| + | PUF | ||
| + | Make multiple PUF biobricks out of existing PUM1 genes and PUM1+endonuclease domain (provided by Dr. Wang at UNC) | ||
| + | 3 ways to test is PUF really binds with the construct | ||
| + | Path 1: Put PUF binding site between the RBS and YFP. If no YFP is detected then PUF has successfully bound. We can also test various PUF binding sites: before the RBS, after the YFP, try using multiple PUF binding sites before the RBS | ||
| + | Suggestion: put the endonuclease site inbetween the RBS and YFP so that when PUF binds we separate the RBS from the reading frame. | ||
| + | Controls: In the positive control, put a PUF binding site, but don’t express PUF. That way we can see that the PUF binding site doesn’t disrupt the YFP expression on it’s own. | ||
| + | Recommendation: This is the immediate and primary focus. | ||
| + | Path 2: PUF between RBS and LacI. If PUF binds then LacI is repressed. The LacI is controlling the YFP, so we now see a modular response to PUF binding. We can also test the different locations of the PUF binding site within this model. | ||
| + | Path 3: UNC has an incomplete project working with a PUF library that recognizes certain genes. We would use the PUF with the endonuclease domain to cut the designated genes. The genes from this library can get made into biobricks. | ||
| + | Details and illustrations of this plan are included in the handout (attached to the meeting minutes). | ||
| + | How are we selling the PUF project? Why are we doing this? Why do people care? | ||
| + | We are creating a research toolkit that uses PUF and linked proteins for translational repression. | ||
| + | This is another technique used to make a gene circuit, but can also be further used in therapeutics, such as treating HIV of myotonic dystrophy. | ||
| + | This puts us in the “new application” category. | ||
| + | It is the hope that this ends up being more effective/faster/cheaper/specific, etc. | ||
| + | We want to sell this project to the judges (both for iGEM and entrepreneurial). | ||
| + | We need to find a current project so we have a specific, concrete example of an awesome application. This is key to market ourselves. We need to show that we have helped solve a problem because PUF was better than any other option available. | ||
| + | 1st step in the entrepreneurship project is to look at patents and trademarks for already existing methods of translational repression. | ||
| + | We need to have a specific need and market to satisfy. Specific examples are key! Data is also good. | ||
| + | Do the simple key wetlab parts of this project, and continually research applications during the summer. | ||
| + | Resveratrol/Piceatannol | ||
| + | Korean lab under Prof. Chul-Ho Yun is willing to share mutant strains with us, and the first author of the paper, Donghyun Kim, is a post-doc in the Kemper lab on campus. Cara is in communication with both the lab and the author | ||
| + | ChemSketch is being used to draw derivatives of piceatannol and then calculate the logP value of that compound to find the solubility. Then use the Molecular operating environment software to model docking of these more soluble compounds into the insulin receptor. Then would turn at least one of the mutant strains (BM3) from the Korean lab into a biobrick. | ||
| + | Resources: The school of Chemical sciences is being contacted about using their software, and the Hergenrother, Spies, Nair, and Cobb labs are also being contacted. 1-2 other iGEMmers will work with Cara on this project. | ||
| + | Recommendations: In vitro production of piceatannol is expensive and time consuming (purify enzyme, etc). But if we pursue a fermentation, we can produce large amounts of piceatannol, which is a very good application and great for our entrepreneurship competition. We could even produce our own reseveratrol to feed into the piceatannol fermentation. Potential problem: can we secrete piceatannol? | ||
| + | Discussion | ||
| + | The PUF project is technique based and the piceatannol project is application based. It’s great that we have both of them and they should be pursued in tandem. | ||
| + | Proposed budget: asking for $21,000 in materials and supplies, but we can get many free supplies. | ||
| + | Don’t worry about money, if we come up with a good wetlab project, then it becomes easier to obtain funding to travel to the actual competition. | ||
| + | We have enough money to do both projects in tandem. | ||
| + | Call Amanda about GeneArt. We may have money to use up with them. | ||
| + | If we check with Courtney before purchasing anything, we should be fine with money. | ||
| + | Once we start doing work, it’s easier to get funding. This is the big idea. | ||
| + | Our budget this year is actually bigger than normal, so as long as we work hard and are responsible, everything should work out. | ||
| + | |||
| + | Voting on Workload | ||
| + | PUF: Angela, Isiah, Uros, Anthony, Adi *, Asha * | ||
| + | Anthony and Isiah, Uros and Asha, Angela and Adi | ||
| + | Phat: Cara, Divya, Bob * | ||
| + | * = have other primary responsibilities within iGEM. Adi is running entrepreneurship, Bob is running the wiki, Asha is running human practices. | ||
| + | Social Event | ||
| + | This Friday, Golden Harbor. Cara will attempt to make reservations. We will meet at Engineering hall at 5:30 to leave on the 5:40 Green. Please be on time! =) | ||
| + | |||
| + | </textarea> | ||
| + | |||
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Revision as of 18:14, 29 June 2012
