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Team:UIUC-Illinois/Notebook/LabNotebook
From 2012.igem.org
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| + | <table dir="ltr" class="tblGenFixed" id="tblMain" border="0" cellpadding="0" cellspacing="0"><tbody><tr class="rShim"><td class="rShim" style="width: 0px;"></td><td class="rShim" style="width: 120px;"></td><td class="rShim" style="width: 351px;"></td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s0">5/29/2012</td><td dir="ltr" class="s1">- Designed 9 primers for LacI. LacZ. and eYFP. Prepared LB.</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s3">- Inoculation of B0014.J23100.wtPUF. B0030.Q04121</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s4">- Transformed the psB1C3 biobrick into DH5a cells</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td></td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s5">5/31/2012</td><td dir="ltr" class="s3">- Transformed C0012, I732005, E0030, I732017. Also transformed PSB1A3, PSB1AK3, PSB1K3. </td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s4">- Inoculated LB media with a psB1C3 colony from the plate grown the day <br>before. </td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s4">- Met with Prof. Bhalerao to talk about PUF application and project direction (RNA scaffolding).</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td></td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s5">6/1/2012</td><td dir="ltr" class="s3">- | ||
| + | Short-term goal changed: make one testing construct, [rbs-puf-eYFP] | ||
| + | cloned into P.protet plasmid with a ptet promoter and terminator </td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s4">- Began inoculation of C0012, I732005, E0030, I732017, PSB1A3, PSB1AK3, <br>PSB1K3. Was incubated for 9 hours, then put in 4 degree room</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td dir="ltr" class="s4">- Inoculated again due to the round-bottom tube being misplaced. Mini-prepped the inoculation.</td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td></td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s5">6/2/2012</td><td dir="ltr" class="s4">- Cultured [PUF+PIN], E0030, and P. protet. designed check primers for [rbs-puf-eYFP] </td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s2"></td><td></td></tr><tr dir="ltr"><td class="hd"><p style="height:16px;">.</p></td><td class="s5">6/3/2012</td><td dir="ltr" class="s4">- Inoculated with a psB1C3 colony in order to prepare a glycerol stock of the strain.</td></tr></tbody></table> | ||
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Revision as of 17:31, 13 July 2012
