Team:UIUC-Illinois/Notebook/LabNotebook

(Difference between revisions)

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Please select a week on the left.

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 1

5/29/2012	- Dr. Ho will ship vectors from Korea on Friday. Should receive them Monday 6/4/12. - Found and read literature on why C4H doesn't express well in E.coli as well as investigating a protocol for expressing the BM3s in E.coli. - Prof Koffas will ship us pUCo-VvSTS-At4CL as one piece if the MTA goes through. - Mark Langston of Octochem has offered to buy some trans-resveratrol for us. p-Courmaric acid is approx $60/25g at TCI America.
5/31/2012	- Group reading over literature - Revised BM3 primer design - Miniprepped 5 cultures: B0030, B0014, Q04121, J23100, WT PUF and put gDNA in -20 temp box
6/1/2012	- Group reading over literature. - Revised BM3 primer design again.
6/2/2012	- Literature review
6/3/2012	- Literature review

Week 2

6/4/2012	- Received BM3 vectors from Prof. Ho as dried samples. - Submitted primer order to Courtney for ordering from IDT. - Literature review. - Planning to extract vector from well-plate.
6/5/2012	- Ordered the part BBa_K122012 from the parts registry, this is 4CL:STS with yeast promoter and terminator. - Informed by the Kim paper, the BM3s are under the control of an IPTG promoter. - Talked to Dr. Rao about making a protocol for testing the BM3s once they are in an expression vector, Dr. Rao wants PHAT group to lyse the cells and try an in vitro assay on the BM3 enzymes first. - Also found the ATCC company sells Rhodobacter capsulatus gDNA which could be useful for PCR of TAL gene. Parts registry does not have a TAL gene for us to order.
6/6/2012	- Read literature about TLC plates. Learned that we can get the plates from the chem store room.
6/7/2012	- Received feedback from Dr. Jin at the advisors meeting. - Will investigate whether TLC plates can separate piceatannol from resveratrol.

Week 3

6/11/2012	- transformed bm3 into dh5a. - investigated ideas with brad.
6/12/2012	- inoculated bm3 wt, bm3 mutant 10, and bm3 mutant 13. 8 ml of each. - investigated ideas with brad.
6/13/2012	- miniprepped bm3 wt, bm3 mutant 10, and bm3 mutant 13. 100ul of each. also note that initial pellet of mutants after centrifuging from media had a slightly grey tint, opposed to the lack of this color in bm3 wt.
6/14/2012	- investigated chemical properties of resveratrol and piceatannol.