Team:UIUC-Illinois

From 2012.igem.org

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<h1>Enzymatic Cascade</h1><br/>
<h1>Enzymatic Cascade</h1><br/>
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<p>In utilizing the versatility of the PUF based toolkit we are developing, our current research involves an engineered metabolic pathway. The characterization and standardization of these genes in biobrick format is underway. Future goals involving the PUF toolkit will focus on tethering enzymes of productive artificial or natural pathways for kinetic favorability.</p>
<p>In utilizing the versatility of the PUF based toolkit we are developing, our current research involves an engineered metabolic pathway. The characterization and standardization of these genes in biobrick format is underway. Future goals involving the PUF toolkit will focus on tethering enzymes of productive artificial or natural pathways for kinetic favorability.</p>
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<h1>PUF</h1><br/>
<h1>PUF</h1><br/>
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<p>This is the PUF protein found on the PUM1 gene in humans. Our main focus and cause of interest in this protein is its ability to recognize single stranded RNA with specificity. The ability to modify PUF with tethered or fused proteins allows for the development of a customizable RNA binding toolkit. Depending on what fusion or tether is used, the functionality of the PUF protein is modified as well.</p>
<p>This is the PUF protein found on the PUM1 gene in humans. Our main focus and cause of interest in this protein is its ability to recognize single stranded RNA with specificity. The ability to modify PUF with tethered or fused proteins allows for the development of a customizable RNA binding toolkit. Depending on what fusion or tether is used, the functionality of the PUF protein is modified as well.</p>
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<h1>RNA scission</h1><br/>
<h1>RNA scission</h1><br/>
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<p>In fusion the PUF protein to a restriction endonuclease, the fusion protein demonstrates the ability to cleave single stranded RNA molecules with customizable specificity up to eight base pairs. Considering this ability, we believe PUF can prove itself as a promising new tool in researching and developing new types of gene therapy as it can silence genes with seqeunces specific to a reseachers interest.  
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<p>In fusion the PUF protein to a restriction endonuclease, the fusion protein demonstrates the ability to cleave single stranded RNA molecules with customizable specificity up to eight base pairs. Considering this ability, we believe PUF can prove itself as a promising new tool in researching and developing new types of gene therapy as it can silence genes with seqeunces specific to a reseachers interest. </p>
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<h1>RNA scaffold utilization</h1><br/>
<h1>RNA scaffold utilization</h1><br/>
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<p>Since PUF has affinity towards RNA molecules, it is possible to synthesize or utilize existing stemloop structures in RNA in order to provide a kinetically favorable platform for tethered proteins to interact. </p>
<p>Since PUF has affinity towards RNA molecules, it is possible to synthesize or utilize existing stemloop structures in RNA in order to provide a kinetically favorable platform for tethered proteins to interact. </p>
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Revision as of 21:09, 22 September 2012

Header

Main

Mission Statement

The ultimate goal of the University of Illinois 2012 iGEM team is to construct an RNA based protein scaffold to use in the optimization of multi-step enzymatic pathways like that involved in the production of Piceatannol from less complex molecules. We aim to improve the versatility and cost efficiency of biological production systems by use of our PUF project RNA scaffold and to prove its viability through the implementation of such a system in our PHAT project's Piceatannol production in E.Coli. Click here to read our project abstract.

Why?

How?

  • Click on a part of our project above you want to read about.

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