Team:UC Chile2/Protocols

From 2012.igem.org

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<h2>Methods for the characterization of Synechocystis PCC 6803 growth curve</h2>
<h2>Methods for the characterization of Synechocystis PCC 6803 growth curve</h2>
This are the methods we used for setting up the growth curve experiment of our Synechocystis PCC 6803 which is further described [[Team:UC_Chile2/Characterization | here ]].
This are the methods we used for setting up the growth curve experiment of our Synechocystis PCC 6803 which is further described [[Team:UC_Chile2/Characterization | here ]].
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<h3>Materials</h3>
<ul>
<ul>
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<li></li>
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<li>4 sterile (autoclaved) 250 mL Erlenmeyer flasks</li>
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<li></li>
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<li>1 liter of BG-11</li>
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<li></li>
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<li>250 mL of BG-110</li>
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<li></li>
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<li>Axenic culture of Synechocystis PCC 6803</li>
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<li>Sterile filtered 1 mL tips</li>
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<li>Spectrophotometer</li>
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<li>5 1 mL Cuvettes</li>
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</ul>
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<br>
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All procedures should be realized under sterile conditions, preferably in a laminar flow hood.
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<ul>
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<li>Measure 150 mL of BG-11 with a sterile 50 mL Falcon tube and put into each of the 3 Erlenmeyer flasks.</li>
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<li>Measure 150 mL of BG-110 with a sterile 50 mL Falcon tube and put into the remaining Erlenmeyer flask.</li>
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<li>Inoculate 1 mL of axenic Synechocystis PCC 6803 with and OD730 of X to each flask (including the one with BG-110). </li>
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<li>Place flasks in rotating incubator and try to avoid leaving them out of the incubator for long when taking measurements</li>
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<li>Measure OD600 and OD730 twice a day (preferably with at least 8 hours of difference between measurements)</li>
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<li>Repeat measurements for 14 days</li>
</ul>
</ul>
</div>
</div>

Revision as of 08:27, 25 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012